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Title: Simple and rapid solid-phase radioimmunoassay for serum progesterone, using the protein A of Staphylococcus aureus as immunoadsorbent

Abstract

A simple, rapid, and inexpensive radioimmunoassay method for serum progesterone is described, which uses a solid-phase technique for separation of antibody-bound from antibody-free progesterone. Rabbit antiprogesterone immunoglobulins are adsorbed on the protein A of formaldehyde- and heat-treated Staphylococcus aureus cells (Pansorbin; Calbiochem-Behring Corp., La Jolla, California). The suspension of antibody-coated Pansorbin retains all its binding activity of 1-2-H(N)-progesterone when kept at + 4/sup 0/ or at -25/sup 0/C for at least 4 months. Dose-response curves obtained with ether-serum extracts and with the progesterone standard do not deviate significantly from parallelism. The progesterone standard gives identical dose-response curves whether diluted in the assay buffer or in a progesterone-free ether-serum extract. The sensitivity of the assay is 0.02 ng/assay tube. The intra-assay variation coefficient is 16%, and the routine interassay variation coefficient is 17%. The mean serum progesterone concentrations were 0.55 ng/ml during the follicular phase of the menstrual cycle and 12.5 ng/ml during the luteal phase. The average blank value for distilled water was 0.02 ng/assay tube.

Authors:
; ;
Publication Date:
Research Org.:
Universite Libre de Bruxelles, Belgium
OSTI Identifier:
6036900
Resource Type:
Journal Article
Journal Name:
J. Lab. Clin. Med.; (United States)
Additional Journal Information:
Journal Volume: 98:1
Country of Publication:
United States
Language:
English
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; PROGESTERONE; RADIOIMMUNOASSAY; DOSE-RESPONSE RELATIONSHIPS; EXPERIMENTAL DATA; IMMUNOGLOBULINS; PROTEINS; RABBITS; STAPHYLOCOCCUS; SUSPENSIONS; ANIMALS; BACTERIA; DATA; DISPERSIONS; GLOBULINS; HORMONES; INFORMATION; ISOTOPE APPLICATIONS; KETONES; MAMMALS; MICROORGANISMS; NUMERICAL DATA; ORGANIC COMPOUNDS; PREGNANES; RADIOASSAY; STEROID HORMONES; STEROIDS; TRACER TECHNIQUES; VERTEBRATES; 550601* - Medicine- Unsealed Radionuclides in Diagnostics

Citation Formats

Jungers, J, Delogne-Desnoeck, J, and Robyn, C. Simple and rapid solid-phase radioimmunoassay for serum progesterone, using the protein A of Staphylococcus aureus as immunoadsorbent. United States: N. p., 1981. Web.
Jungers, J, Delogne-Desnoeck, J, & Robyn, C. Simple and rapid solid-phase radioimmunoassay for serum progesterone, using the protein A of Staphylococcus aureus as immunoadsorbent. United States.
Jungers, J, Delogne-Desnoeck, J, and Robyn, C. 1981. "Simple and rapid solid-phase radioimmunoassay for serum progesterone, using the protein A of Staphylococcus aureus as immunoadsorbent". United States.
@article{osti_6036900,
title = {Simple and rapid solid-phase radioimmunoassay for serum progesterone, using the protein A of Staphylococcus aureus as immunoadsorbent},
author = {Jungers, J and Delogne-Desnoeck, J and Robyn, C},
abstractNote = {A simple, rapid, and inexpensive radioimmunoassay method for serum progesterone is described, which uses a solid-phase technique for separation of antibody-bound from antibody-free progesterone. Rabbit antiprogesterone immunoglobulins are adsorbed on the protein A of formaldehyde- and heat-treated Staphylococcus aureus cells (Pansorbin; Calbiochem-Behring Corp., La Jolla, California). The suspension of antibody-coated Pansorbin retains all its binding activity of 1-2-H(N)-progesterone when kept at + 4/sup 0/ or at -25/sup 0/C for at least 4 months. Dose-response curves obtained with ether-serum extracts and with the progesterone standard do not deviate significantly from parallelism. The progesterone standard gives identical dose-response curves whether diluted in the assay buffer or in a progesterone-free ether-serum extract. The sensitivity of the assay is 0.02 ng/assay tube. The intra-assay variation coefficient is 16%, and the routine interassay variation coefficient is 17%. The mean serum progesterone concentrations were 0.55 ng/ml during the follicular phase of the menstrual cycle and 12.5 ng/ml during the luteal phase. The average blank value for distilled water was 0.02 ng/assay tube.},
doi = {},
url = {https://www.osti.gov/biblio/6036900}, journal = {J. Lab. Clin. Med.; (United States)},
number = ,
volume = 98:1,
place = {United States},
year = {Wed Jul 01 00:00:00 EDT 1981},
month = {Wed Jul 01 00:00:00 EDT 1981}
}