Chicken embryo fibroblasts exposed to weak, time-varying magnetic fields share cell proliferation, adenosine deaminase activity, and membrane characteristics of transformed cells

Journal Article · Fri Jan 01 00:00:00 EST 1993 · Bioelectromagnetics (New York); (United States)

DOI:https://doi.org/10.1002/bem.2250140305· OSTI ID:6027706

Parola, A H; Porat, N; Kiesow, L A ^[1]

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1. Naval Medical Research Institute, Bethesda, MD (United States)

Chicken embryo fibroblasts (CEF) exposed to a sinusoidally varying magnetic field (SVMF) (100 Hz, 700 microT, for 24 h) showed a remarkable rise of segmental rotational relaxation rate of adenosine deaminase (ADA, EC 3.5.4.4) as determined by multifrequency phase fluorometry. Pyrene-labeled, small subunit ADA was applied to cultured (normal) CEF, which have available and abundant ADA complexing protein (ADCP) on their plasma membranes. Sine-wave-modulated fluorometry of the pyrene yielded a profile of phase angle vs. modulation frequency. In SVMF-treated cells and in Rous-sarcoma-virus (RSV) transformed cells the differential phase values at low modulation frequencies of the excitation are remarkably reduced. This effect is magnetic rather than thermal, because the temperature was carefully controlled and monitored; nevertheless to further check this matter we studied CEF, infected by the RSV-Ts68 temperature-sensitive mutant (36 degrees C transformed, 41 degrees C revertant). When grown at 36 degrees C in the SVMF, cells did not show the slightest trend towards reversion, as would be expected had there been local heating. Concomitant with the increased segmental rotational relaxation rate of ADA, there was a decrease in fluorescence lifetime and a slight, yet significant, increase in membrane lipid microfluidity. These biophysical observations prompted us to examine the effect of SVMF on cell proliferation and ADA activity (a malignancy marker): higher rates of cell proliferation and reduced specific activity of ADA were observed.

• OSTI ID: 6027706
• Journal Information: Bioelectromagnetics (New York); (United States), Vol. 14:3; ISSN 0197-8462
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
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ADENOSINE
BIOLOGICAL INDICATORS
ENZYME ACTIVITY
ELECTROMAGNETIC FIELDS
BIOLOGICAL EFFECTS
FIBROBLASTS
CELL PROLIFERATION
CELL TRANSFORMATIONS
CELL DIVISION
CHICKENS
ENVIRONMENTAL EXPOSURE
FLUORESCENCE SPECTROSCOPY
ANIMAL CELLS
ANIMALS
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FOWL
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NUCLEOTIDES
ORGANIC COMPOUNDS
RIBOSIDES
SOMATIC CELLS
SPECTROSCOPY
VERTEBRATES
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550300 - Cytology