Nucleotide sequence of Bacillus subtilis dnaB: a gene essential for DNA replication initiation and membrane attachment
The complete nucleotide sequence of the Bacillus subtilis dnaB gene and its flanking regions was determined. The dnaB gene is essential for both replication initiation and membrane attachment of the origin region of the chromosome and plasmid pUB110. It has been known that there are two different classes (dnaBI and dnaBII) in the dnaB mutants; dnaBI is essential for both chromosome and pUB110 replication, whereas dnaBII is necessary only for chromosome replication. The nucleotide sequence revealed that dnaBI and dnaBII are two functional domains in the single dnaB gene. The mutation sites of two mutants, belonging to dnaBI and dnaBII, respectively, were also determined as substitutions of amino acids. The putative DnaB protein deduced from nucleotide sequence consists of 472 amino acids (55 kDa) with no cysteine residue. A 55-kDa polypeptide produced in an in vitro transcription-translation system was labeled with (/sup 35/S)methionine but not with (/sup 35/S)cysteine. The DnaB protein has a highly hydrophobic sequence of 20 amino acids in its N-terminal region, a possible DNA binding site, and two possible ATP binding sites. The dnaBI domain is between the DNA binding site and one of the ATP binding sites; the dnaBII domain is close to the other ATP binding site. Comparison of the amino acid sequence between the dnaB protein and those of other dna genes of Escherichia coli showed no homology, suggesting that the dnaB gene of B, subtilis may be analogous to a hitherto undiscovered gene in E. coli.
- Research Organization:
- Univ. of Colorado, Boulder, CO (United States)
- OSTI ID:
- 6023017
- Journal Information:
- Proc. Natl. Acad. Sci. U.S.A.; (United States), Vol. 84:3
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
62 RADIOLOGY AND NUCLEAR MEDICINE
BACILLUS SUBTILIS
DNA SEQUENCING
LABELLED COMPOUNDS
BIOSYNTHESIS
POLYPEPTIDES
AMINO ACID SEQUENCE
AUTORADIOGRAPHY
CYSTEINE
DNA
DNA REPLICATION
GENES
METHIONINE
SULFUR 35
TRANSCRIPTION
AMINO ACIDS
BACILLUS
BACTERIA
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBOXYLIC ACIDS
DAYS LIVING RADIOISOTOPES
DRUGS
EVEN-ODD NUCLEI
ISOTOPES
LIGHT NUCLEI
LIPOTROPIC FACTORS
MICROORGANISMS
MOLECULAR STRUCTURE
NUCLEI
NUCLEIC ACID REPLICATION
NUCLEIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PEPTIDES
PROTEINS
RADIOISOTOPES
STRUCTURAL CHEMICAL ANALYSIS
SULFUR ISOTOPES
SYNTHESIS
THIOLS
550201* - Biochemistry- Tracer Techniques
550601 - Medicine- Unsealed Radionuclides in Diagnostics