Cloning and characterization of human liver cDNA encoding a protein S precursor
Human liver cDNA encoding a protein S precursor was isolated from two cDNA libraries by two different techniques. Based upon the frequency of positive clones, the abundance of mRNA for protein S is approx. = 0.01%. Blot hybridization of electrophoretically fractionated poly(A)/sup +/ RNA revealed a major mRNA approx. = 4 kilobases long and two minor forms of approx. = 3.1 and approx. = 2.6 kilobases. One of the cDNA clones contains a segment encoding a 676 amino acid protein S precursor, as well as 108 and 1132 nucleotides of 5' and 3' noncoding sequence, respectively, plus a poly(A) region at the 3' end. The cDNAs are adenosine plus thymidine-rich (60%) except for the 5' noncoding region, where 78% of the nucleotides are guanosine or cytosine. The protein precursor consists of a 41 amino acid leader peptide followed by 635 amino acids corresponding to mature protein S. Comparison of the mature protein region with homologous vitamin K-dependent plasma proteins shows that it is composed of the following domains: an amino-terminal ..gamma..-carboxyglutamic acid-rich region of 37 amino acids; a 36 amino acid linker region rich in hydroxy amino acids; four epidermal growth factor-like segments, each approx. = 45 amino acids long; and a 387 amino acid carboxyl-terminal domain of unrecognized structure and unknown function.
- Research Organization:
- Lilly Research Labs., Indianapolis, IN
- OSTI ID:
- 6022875
- Journal Information:
- Proc. Natl. Acad. Sci. U.S.A.; (United States), Vol. 84:2
- Country of Publication:
- United States
- Language:
- English
Similar Records
Cloning of the. cap alpha. chain of human platelet glycoprotein Ib: a transmembrane protein with homology to leucine-rich. cap alpha. /sub 2/-glycoprotein
Cloning and sequencing of cDNAs encoding. cap alpha. and. beta. subunits of human pyruvate dehydrogenase
Related Subjects
RECOMBINANT DNA
DNA SEQUENCING
DNA-CLONING
BLOOD COAGULATION
GROWTH
HYBRIDIZATION
LIVER
MAN
MESSENGER-RNA
PROTEINS
ANIMALS
BODY
CLONING
DIGESTIVE SYSTEM
DNA
GLANDS
MAMMALS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PRIMATES
RNA
STRUCTURAL CHEMICAL ANALYSIS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques