Epitopes of proteoglycans eliciting an anti-proteoglycan response in chronic immune synovitis

Journal Article · Sun Feb 01 00:00:00 EST 1987 · Proc. Natl. Acad. Sci. U.S.A.; (United States)

DOI:https://doi.org/10.1073/pnas.84.3.832· OSTI ID:6021511

Yoo, J U; Kresina, T F; Malemud, C J; Goldberg, V M

This study details the immune response to cartilage proteoglycan in experimental chronic IgG-induced immune synovitis. With the use of radioimmunoassay, antibodies reactive with purified rabbit proteoglycan monomer were observed in nine of nine rabbits with immune synovitis. IgG-immunized but nonsynovitic control animals with no pathology showed no antibody response. A panel of murine monoclonal antibodies with defined specificity towards rabbit proteoglycan were utilized to characterize the epitope specificity of the immune synovitis polyclonal anti-proteoglycan response. One murine monoclonal antibody, 6C11, inhibited the binding of the polyclonal antisera to proteoglycan in all nine animals with significant (>40/sup 5/) inhibition in six of nine rabbits. Further inhibition studies utilizing DEAE-cellulose-resolved proteoglycan tryptic peptides revealed that peptides poor in chondroitin sulfate were strong inhibitors of binding of the polyclonal antibodies to the proteoglycan substrate. In particular, keratan sulfate-containing tryptic peptides were most inhibitory on a per weight basis. These results indicate that, in chromic IgG-induced immune synovitis, anti-proteoglycan antibodies elicited are heterogeneous with regard to specificity, but a relatively large proportion predominantly recognized a portion of the proteoglycan molecule containing core protein and associated keratan sulfate.

• Research Organization: Case Western Reserve Univ., Cleveland, OH
• OSTI ID: 6021511
• Journal Information: Proc. Natl. Acad. Sci. U.S.A.; (United States), Vol. 84:3
• Country of Publication: United States
• Language: English

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