Biodegradation of azaarenes and creosote in aqueous and organic liquid phase immobilized cell bioreactors by bacteria isolated from creosote contaminated soil
The biodegradation of azaarenes and coal-tar creosote was studied using aerobic bacteria isolated from creosote contaminated soil as inocula in batch cultures and in immobilized cell bioreactors. Biodegradation of quinoline, isoquinoline, and 6-methylquinoline by pure and mixed cultures yielded mono-hydroxylated metabolites as the primary products of azaarene metabolism. All azaarene degrading cultures could degrade quinoline, suggesting a common metabolic pathway based on quinoline metabolism. Mixed cultures attacking creosote degraded 2- and 3-ring polyaromatic hydrocarbons and heterocycles, but were unable to degrade 4- and 5-ring PAH. The degradation rate and loading capacity for quinoline was greatly enhanced in the bioreactors in comparison to batch cultures. The rates of isoquinoline, 6-methylquinoline degrading strain of Pseudomonas putida successfully removed 6-methylquinoline from solution in decane in a water-limited, non-aqueous liquid phase immobilized cell bioreactor. These experiments demonstrate the ability of environmental organisms to biodegrade several biologically active compounds under conditions suitable for bioremediation applications.
- Research Organization:
- Louisville Univ., KY (United States)
- OSTI ID:
- 6021265
- Resource Relation:
- Other Information: Thesis (Ph.D.)
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AZAARENES
BIODEGRADATION
CREOSOTE
SOILS
CONTAMINATION
AEROBIC CONDITIONS
BACTERIA
BIOREACTORS
COAL TAR
HYDROCARBONS
PSEUDOMONAS
REMEDIAL ACTION
AROMATICS
CHEMICAL REACTIONS
DECOMPOSITION
HETEROCYCLIC COMPOUNDS
MICROORGANISMS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OTHER ORGANIC COMPOUNDS
TAR
020900* - Petroleum- Environmental Aspects
560300 - Chemicals Metabolism & Toxicology