Comparison of oxalate formation from ascorbic and glyoxyl acids in detached glandular heads of tobacco trichomes
Ca-oxalate crystal containing cells from detached glandular heads of trichomes from Nicotiana tabacum, TI 1068, are capable of converting (1-/sup 14/C) ascorbic acid (AA) and (1-/sup 14/C) glyoxylic acid (GA) to oxalate. AA was found to be a better precursor for oxalate formation than GA. In detached glandular heads, 3.6x more label was converted to oxalate from AA than from GA, in the epidermis the factor was 3x while that with petiole tissue was 7x. Oxalate formation from AA, in detached glandular heads, was only partially inhibited in the dark and in the presence of metabolic inhibitors, suggesting that a nonenzymatic component might be involved. Oxalate formation from GA increased in the presence of metabolic inhibitors. During treatment of detached glandular heads with 2 mM Ca-acetate for 2 days, oxalate formation from AA was stimulated 3 fold, while the presence of 2mM Ca-acetate had no effect on the oxalate formation from GA. These results suggest that Ca/sup 2 +/ stimulates the formation of Ca-oxalate crystals in glandular head cells, and that AA can serve as a precursor for oxalate production.
- Research Organization:
- Univ. of Kentucky, Lexington
- OSTI ID:
- 6021137
- Report Number(s):
- CONF-8707108-; TRN: 87-037434
- Journal Information:
- Plant Physiol., Suppl.; (United States), Vol. 83:4; Conference: Annual meeting of the American Society of Plant Physiologists, St. Louis, MO, USA, 19 Jul 1987
- Country of Publication:
- United States
- Language:
- English
Similar Records
Trichomes of tobacco excrete zinc as zinc-substituted calciumcarbonate and other zinc-containing compounds
Characterization of a Unique Pair of Ferredoxin and Ferredoxin NADP+ Reductase Isoforms That Operates in Non-Photosynthetic Glandular Trichomes
Related Subjects
CALCIUM
BIOLOGICAL FUNCTIONS
OXALATES
BIOSYNTHESIS
ANIONS
ASCORBIC ACID
CARBON 14 COMPOUNDS
GLYOXYLIC ACID
METABOLISM
PLANT TISSUES
PRECURSOR
TOBACCO
TRACER TECHNIQUES
ALDEHYDES
ALKALINE EARTH METALS
BODY
CARBOXYLIC ACID SALTS
CARBOXYLIC ACIDS
CHARGED PARTICLES
ELEMENTS
FUNCTIONS
IONS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
METALS
ORGANIC ACIDS
ORGANIC COMPOUNDS
SYNTHESIS
TISSUES
VITAMINS
551001* - Physiological Systems- Tracer Techniques