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Title: Replication-defective vectors of reticuloendotheliosis virus transduce exogenous genes into somatic stem cells of the unincubated chicken embryo

Abstract

Replication-defective vectors derived from reticuloendotheliosis virus were used to transduce exogenous genes into early somatic stem cells of the chicken embryo. One of these vectors transduced and expressed the chicken growth hormone coding sequence. The helper cell line, C3, was used to generate stocks of vector containing about 10/sup 4/ transducing units per ml. Injection of 5- to 20-..mu..l volumes of vector directly beneath the blastoderm of unincubated chicken embryos led to infection of somatic stem cells. Infected embryos and adults contained unrearranged integrated proviral DNAs. Embryos expressed the transduced chicken growth hormone gene and contained high levels of serum growth hormone. Blood, brain, muscle, testis, and semen contained from individuals injected as embryos contained vector DNA. Replication-defective vectors of the reticuloendotheliosis virus transduced exogenous genes into chicken embryonic stem cells in vivo.

Authors:
; ; ; ; ; ; ; ; ;
Publication Date:
Research Org.:
Amgen Inc., Thousand Oaks, CA (USA)
OSTI Identifier:
6017506
Resource Type:
Journal Article
Journal Name:
J. Virol.; (United States)
Additional Journal Information:
Journal Volume: 63:6
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; 59 BASIC BIOLOGICAL SCIENCES; CHICKENS; GENETIC ENGINEERING; DISEASE VECTORS; BIOLOGICAL FUNCTIONS; STH; GENES; DNA; EMBRYOS; GENE REGULATION; IN VIVO; STEM CELLS; VIRAL DISEASES; VIRUSES; ANIMAL CELLS; ANIMALS; BIRDS; DISEASES; FOWL; FUNCTIONS; HORMONES; INFECTIOUS DISEASES; MICROORGANISMS; NUCLEIC ACIDS; ORGANIC COMPOUNDS; PARASITES; PEPTIDE HORMONES; PITUITARY HORMONES; SOMATIC CELLS; VERTEBRATES; 140504* - Solar Energy Conversion- Biomass Production & Conversion- (-1989); 550900 - Pathology

Citation Formats

Bosselman, R A, Hsu, R Y, Boggs, T, Hu, S, Bruszewski, J, Ou, S, Souza, L, Kozar, L, Martin, F, and Nicolson, M. Replication-defective vectors of reticuloendotheliosis virus transduce exogenous genes into somatic stem cells of the unincubated chicken embryo. United States: N. p., 1989. Web.
Bosselman, R A, Hsu, R Y, Boggs, T, Hu, S, Bruszewski, J, Ou, S, Souza, L, Kozar, L, Martin, F, & Nicolson, M. Replication-defective vectors of reticuloendotheliosis virus transduce exogenous genes into somatic stem cells of the unincubated chicken embryo. United States.
Bosselman, R A, Hsu, R Y, Boggs, T, Hu, S, Bruszewski, J, Ou, S, Souza, L, Kozar, L, Martin, F, and Nicolson, M. 1989. "Replication-defective vectors of reticuloendotheliosis virus transduce exogenous genes into somatic stem cells of the unincubated chicken embryo". United States.
@article{osti_6017506,
title = {Replication-defective vectors of reticuloendotheliosis virus transduce exogenous genes into somatic stem cells of the unincubated chicken embryo},
author = {Bosselman, R A and Hsu, R Y and Boggs, T and Hu, S and Bruszewski, J and Ou, S and Souza, L and Kozar, L and Martin, F and Nicolson, M},
abstractNote = {Replication-defective vectors derived from reticuloendotheliosis virus were used to transduce exogenous genes into early somatic stem cells of the chicken embryo. One of these vectors transduced and expressed the chicken growth hormone coding sequence. The helper cell line, C3, was used to generate stocks of vector containing about 10/sup 4/ transducing units per ml. Injection of 5- to 20-..mu..l volumes of vector directly beneath the blastoderm of unincubated chicken embryos led to infection of somatic stem cells. Infected embryos and adults contained unrearranged integrated proviral DNAs. Embryos expressed the transduced chicken growth hormone gene and contained high levels of serum growth hormone. Blood, brain, muscle, testis, and semen contained from individuals injected as embryos contained vector DNA. Replication-defective vectors of the reticuloendotheliosis virus transduced exogenous genes into chicken embryonic stem cells in vivo.},
doi = {},
url = {https://www.osti.gov/biblio/6017506}, journal = {J. Virol.; (United States)},
number = ,
volume = 63:6,
place = {United States},
year = {Thu Jun 01 00:00:00 EDT 1989},
month = {Thu Jun 01 00:00:00 EDT 1989}
}