A simple, sensitive, and accurate alcohol electrode

Journal Article · Fri Apr 01 00:00:00 EST 1983 · Biotechnol. Bioeng.; (United States)

DOI:https://doi.org/10.1002/bit.260250413· OSTI ID:6015861

Verduyn, C; Scheffers, W A; Van Dijken, J P

The construction and performance of an enzyme electrode is described which specifically detects lower primary aliphatic alcohols in aqueous solutions. The electrode consists of a commercial Clark-type oxygen electrode on which alcohol oxidase (E.C. 1.1.3.13) and catalase were immobilized. The decrease in electrode current is linearly proportional to ethanol concentrations betwee 1 and 25 ppm. The response of the electrode remains constant during 400 assays over a period of two weeks. The response time is between 1 and 2 min. Assembly of the electrode takes less than 1 h.

• Research Organization: Delft University of Technology, Laboratory of Microbiology, Julianalaan 67a, 2628 BC Delft
• OSTI ID: 6015861
• Journal Information: Biotechnol. Bioeng.; (United States), Vol. XXV:4
• Country of Publication: United States
• Language: English

Related Subjects

10 SYNTHETIC FUELS
37 INORGANIC
ORGANIC
PHYSICAL AND ANALYTICAL CHEMISTRY
ETHANOL
BIOASSAY
DETECTION
CATALASE
ELECTRODES
IMMOBILIZED ENZYMES
OXIDOREDUCTASES
PRODUCTION
ALCOHOLS
CARBOXYLIC ACIDS
ENZYMES
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PEROXIDASES
PORPHYRINS
090100* - Hydrocarbon Fuels- (-1989)
400400 - Electrochemistry