(Structure and stability of nucleic acids)
We have studied the conformations of DNA and RNA oligonucleotides in order to relate their structures to their biological roles. We have discovered a novel conformation for a guanine-rich sequence which occurs in telomeres. This finding of one biologically relevant non-Watson-Crick base pair suggests that other non-standard pairs may be important. We have continued a systematic study of the thermodynamics of base pair formation in double stranded DNA. We have established the conformation and thermodynamics of a 3 in. viral RNA structural element. We have made a direct comparison between the thermodynamic stability of mismatched bases and the kinetics of misincorporation of bases by DNA polymerase. Drosophila spermatogenesis and sickle hemoglobin polymerization have been studied with the differential polarization microscope, which was invented and developed in this laboratory. Left-handed Z-RNA, discovered in this laboratory in 1984, has been characterized by NMR, circular dichroism and Raman scattering. (MHB)
- Research Organization:
- California Univ., Berkeley, CA (USA). Dept. of Chemistry
- Sponsoring Organization:
- USDOE; USDOE, Washington, DC (USA)
- DOE Contract Number:
- FG03-86ER60406
- OSTI ID:
- 6012403
- Report Number(s):
- DOE/ER/60406-T3; ON: DE91008274
- Country of Publication:
- United States
- Language:
- English
Similar Records
[Structure and stability of nucleic acids]. Progress report, July 1, 1988--June 30, 1991
Monovalent cation induced structural transitions in telomeric DNAs: G-DNA folding intermediates
Related Subjects
DNA
STRUCTURE-ACTIVITY RELATIONSHIPS
OLIGONUCLEOTIDES
RNA
DICHROISM
DNA HYBRIDIZATION
DNA MISMATCH
DNA POLYMERASES
DNA REPLICATION
DROSOPHILA
HEMOGLOBIN
HYPOXANTHINE
MOLECULAR STRUCTURE
NMR SPECTRA
NUCLEOLI
NUCLEOTIDES
OPTICAL MICROSCOPY
POLARIZATION
POLYMERIZATION
PROGRESS REPORT
RAMAN SPECTROSCOPY
REACTION KINETICS
SPERMATOGENESIS
THERMODYNAMICS
ANIMALS
AROMATICS
ARTHROPODS
AZAARENES
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CHEMICAL REACTIONS
DIPTERA
DOCUMENT TYPES
ENZYMES
FLIES
FRUIT FLIES
GAMETOGENESIS
GLOBINS
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HYBRIDIZATION
HYDROXY COMPOUNDS
INSECTS
INVERTEBRATES
KINETICS
LASER SPECTROSCOPY
MICROSCOPY
NUCLEIC ACID REPLICATION
NUCLEIC ACIDS
NUCLEOTIDYLTRANSFERASES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHOSPHORUS-GROUP TRANSFERASES
PIGMENTS
POLYMERASES
PORPHYRINS
PROTEINS
PURINES
SPECTRA
SPECTROSCOPY
TRANSFERASES
550200* - Biochemistry