Preparation and characterization of. beta. -D-glucosidase immobilized in calcium alginate

Conference · Fri Jan 01 00:00:00 EST 1982

OSTI ID:6011936

Woodward, J; Krasniak, S R; Smith, R D; Spielberg, F; Zachry, G S

This study investigated the immobilization of ..beta..-D-glucosidase (E.C. 3.2.1.21) in calcium alginate gel spheres. The immobilized enzyme catalyzed the hydrolysis of cellobiose to glucose. During preparation of the enzyme-containing spheres, 49% of the initial activity was lost from the alginate slurry. There was a 37% retention of the enzyme activity that was actually immobilized within the spheres. This loss of activity upon immobilization may be caused by inhibition of the enzyme by calcium cations and alginate anions present in the gel. Mass transfer effects were apparently minimal in this system and were not responsible for the activity loss. Leakage of the enzyme from the spheres occurred during storage of the spheres occurred during storage of the spheres at 4/sup 0/C and during their incubation with stirring at 23/sup 0/C. Leakage was severe at pH 5.0 but could be prevented if the enzyme was treated with glutaraldehyde prior to immobilization.

• Research Organization: Oak Ridge National Lab., TN (USA)
• DOE Contract Number: W-7405-ENG-26
• OSTI ID: 6011936
• Report Number(s): CONF-820580-6; ON: DE83012587
• Resource Relation: Conference: 4. symposium on biotechnology in energy production and conservation, Gatlinburg, TN, USA, 11 May 1982
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC
ORGANIC
PHYSICAL AND ANALYTICAL CHEMISTRY
59 BASIC BIOLOGICAL SCIENCES
GLUCOSIDASE
CATALYTIC EFFECTS
GELATION
ALGINIC ACID
CALCIUM COMPOUNDS
CARBOHYDRATES
CELLULOSE
CHEMICAL PREPARATION
GELS
GLUCOSE
HYDROLYSIS
IMMOBILIZED ENZYMES
SPHERES
STORAGE
ALDEHYDES
ALKALINE EARTH METAL COMPOUNDS
CHEMICAL REACTIONS
COLLOIDS
DECOMPOSITION
DISPERSIONS
ENZYMES
GLYCOSYL HYDROLASES
HEXOSES
HYDROLASES
LYSIS
MONOSACCHARIDES
O-GLYCOSYL HYDROLASES
ORGANIC COMPOUNDS
POLYSACCHARIDES
SACCHARIDES
SOLVOLYSIS
SYNTHESIS
400301* - Organic Chemistry- Chemical & Physicochemical Properties- (-1987)
550200 - Biochemistry