Adenosine transport systems on dissociated brain cells from mouse, guinea-pig, and rat
- Univ. of Manitoba, Winnipeg (Canada)
The kinetics and sodium dependence of adenosine transport were determined using an inhibitor-stop method on dissociated cell body preparations obtained from mouse, guinea-pig and rat brain. Transport affinity (KT) values for the high affinity adenosine transport systems KT(H) were significantly different between these three species; mean +/- SEM values were 0.34 +/- 0.1 in mouse, 0.9 +/- 0.2 in rat, and 1.5 +/- 0.5 microM in guinea-pig. The KT values for the low affinity transport system KT(L) were not different between the three species. Brain cells from rat displayed a significantly greater maximal capacity to accumulate (3H)adenosine (Vmax) than did mouse or guinea-pig for the high affinity system, or than did mouse for the low affinity system. When sodium chloride was replaced in the transport medium with choline chloride, the KT(H) values for guinea-pig and rat were both increased by approximately 100%; only in rat did the change reach statistical significance. The sodium-dependence of adenosine transport in mouse brain was clearly absent. The differences between KT(H) values in mouse and those in guinea-pig or rat were accentuated in the absence of sodium. The differences in kinetic values, ionic requirements, and pharmacological characteristics between adenosine transporters in CNS tissues of mouse, guinea-pig and rat may help account for some of the variability noted among species in terms of their physiological responses to adenosine.
- OSTI ID:
- 6011788
- Journal Information:
- Neurochemical Research; (USA), Vol. 15:9; ISSN 0364-3190
- Country of Publication:
- United States
- Language:
- English
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ADENOSINE
MEMBRANE TRANSPORT
AFFINITY
BRAIN
CELL MEMBRANES
GENETIC VARIABILITY
GUINEA PIGS
MICE
RATS
REGRESSION ANALYSIS
SODIUM COMPOUNDS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ALKALI METAL COMPOUNDS
ANIMALS
BIOLOGICAL VARIABILITY
BODY
CELL CONSTITUENTS
CENTRAL NERVOUS SYSTEM
HYDROGEN COMPOUNDS
ISOTOPE APPLICATIONS
MAMMALS
MATHEMATICS
MEMBRANES
NERVOUS SYSTEM
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANS
RIBOSIDES
RODENTS
STATISTICS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques