Development and application of a radioimmunoassay for physostigmine
Physostigmine, an alkaloid obtained from the Calibar bean, is a cholinergic drug that has been used for memory enhancement in Alzheimer's disease for management of glaucoma and to counter tricyclic poisoning. In addition, military organizations in several countries have an active interest in physostigmine as a pretreatment compound for nerve agent poisoning. Although physostigmine has been used for many years, there has not been a simple sensitive assay to measure low plasma levels associated with therapeutic dosages. Available methods include HPLC assays and capillary column chromatography using chemical ionization mass spectrometry. All of these methods have a number of shortcomings (poor sensitivity, large sample size and time consuming sample preparation) that can be solved with a RIA. We have been able to prepare antibodies to physostigmine that are specific and have a good binding affinity. With these antibodies we have developed a sensitive RIA for physostigmine.
- Research Organization:
- Army Medical Research Inst. of Chemical Defense, Aberdeen Proving Ground, MD (USA)
- OSTI ID:
- 6010882
- Report Number(s):
- AD-A-228432/1/XAB; USAMRICD-P-89-005; CNN: DAMD17-84-C-4235
- Resource Relation:
- Other Information: Pub. in The Jnl. of Pharmacology and Experimental Therapeutics, Vol. 251, No. 2, 606-611(1989)
- Country of Publication:
- United States
- Language:
- English
Similar Records
Acetylcholinesterase inhibition and anti-Soman efficacy of homologs of physostigmine. (Reannouncement with new availability information)
Pre- and post-treatment effect of physostigmine on soman-inhibited human erythrocyte and muscle acetylcholinesterase in vitro
Related Subjects
45 MILITARY TECHNOLOGY, WEAPONRY, AND NATIONAL DEFENSE
ALKALOIDS
RADIOIMMUNOASSAY
CHEMICAL WARFARE AGENTS
CHEMOTHERAPY
NERVOUS SYSTEM DISEASES
BIOASSAY
DIAGNOSTIC TECHNIQUES
DISEASES
IMMUNOASSAY
IMMUNOLOGY
ISOTOPE APPLICATIONS
ORGANIC COMPOUNDS
RADIOASSAY
RADIOIMMUNODETECTION
RADIOIMMUNOLOGY
THERAPY
TRACER TECHNIQUES
WEAPONS
550201* - Biochemistry- Tracer Techniques
450600 - Military Technology
Weaponry
& National Defense- Chemical & Biological- (1990)