Detection and quantification of 8-hydroxydeoxyguanosine adducts in peripheral blood of people exposed to ionizing radiation
- Univ. of Colorado Health Sciences Center, Denver (United States)
- National Cancer Inst., Bethesda, MD (United States)
- Christie Hospital and Radium Inst., Manchester (United Kingdom)
Ionizing radiation produces a variety of damaging insults to nucleic acids, including the promutagenic lesion 8-hydroxydeoxyguanosine. In the present study, the 8-hydroxydeoxyguanosine content of peripheral blood leukocyte DNA isolated from individuals exposed to therapeutic doses of ionizing radiation was determined by a HPLC-coupled [sup 32]P-postlabeling assay. Peripheral blood leukocyte DNA from individuals irradiated with 180--200 cGy were observed to contain 2--4.5 times as much 8-hydroxydeoxyguanosine as that from unexposed individuals. These results were confirmed by the use of a HPLC-coupled electrochemical detection system. Thus, human exposure to ionizing radiation significantly increased the circulating leukocyte DNA content of 8-hydroxydeoxyguanosine. 11 refs., 2 figs., 1 tab.
- OSTI ID:
- 6005943
- Report Number(s):
- CONF-9110511-; CODEN: EVHPAZ; TRN: 93-020784
- Journal Information:
- Environmental Health Perspectives; (United States), Vol. 99; Conference: Symposium on biomonitoring and susceptibility markers in human cancer: applications in molecular epidemiology and risk assessment, Kailua-Kona, HI (United States), 26 Oct - 1 Nov 1991; ISSN 0091-6765
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
DNA ADDUCTS
DETECTION
QUANTITATIVE CHEMICAL ANALYSIS
IONIZING RADIATIONS
BIOLOGICAL EFFECTS
LEUKOCYTES
BIOASSAY
ANTIOXIDANTS
NUCLEIC ACIDS
PHOSPHORUS 32
RADICALS
ADDUCTS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CHEMICAL ANALYSIS
DAYS LIVING RADIOISOTOPES
ISOTOPES
LIGHT NUCLEI
MATERIALS
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PHOSPHORUS ISOTOPES
RADIATIONS
RADIOISOTOPES
560151* - Radiation Effects on Animals- Man
550200 - Biochemistry