Indigenous and enhanced mineralization of pyrene, benzo(a)pyrene, and carbazole in soils
- Univ. of Cincinnati, OH (United States)
The authors studied the mineralization of pyrene, carbazole, and benzo(a)pyrene in soils obtained from three abandoned coal gasification plants in southern Illinois. The soils had different histories of past exposure to hydrocarbon contamination and different amounts of total organic carbon, microbial biomass, and microbial activity. Mineralization was measured by using serum bottle radiorespirometry. The levels of indigenous mineralization of {sup 14}C-labeled compounds ranged from 10 to 48% for pyrene, from undetectable to 46% for carbazole, and from undetectable to 25% for benzo(a)pyrene following long-term (<180-day) incubations. Pyrene and carbazole were degraded with short or no lag periods in all soils, but benzo(a)pyrene mineralization occurred after a 28-day lag period. Mineralization was not dependent on high levels of microbial biomass and activity in the soils. Bacterial cultures that were capable of degrading pyrene and carbazole were isolated by enrichment, grown in pure culture, and reintroduced into soils. Reintroduction of a pyrene-degrading bacterium enhanced mineralization to a level of 55% within 2 days, compared with a level of 1% for the indigenous population. The carbazole degrader enhanced mineralization to a level of 45% after 7 days in a soil that showed little indigenous carbazole mineralization. The pyrene and carbazole degraders which they isolated were identified as a Mycobacterium sp. and Xanthamonas sp., respectively.
- OSTI ID:
- 6003808
- Journal Information:
- Applied and Environmental Microbiology; (United States), Vol. 57:12; ISSN 0099-2240
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
01 COAL, LIGNITE, AND PEAT
BENZOPYRENE
BIODEGRADATION
CARBAZOLES
PYRENE
ABANDONED SITES
CARBON 14 COMPOUNDS
COAL GASIFICATION PLANTS
ILLINOIS
SOILS
TRACER TECHNIQUES
AROMATICS
AZAARENES
AZOLES
CARBON COMPOUNDS
CHEMICAL REACTIONS
CONDENSED AROMATICS
DECOMPOSITION
DEVELOPED COUNTRIES
FEDERAL REGION V
HETEROCYCLIC COMPOUNDS
HYDROCARBONS
INDUSTRIAL PLANTS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
NORTH AMERICA
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
USA
540220* - Environment
Terrestrial- Chemicals Monitoring & Transport- (1990-)
010900 - Coal
Lignite
& Peat- Environmental Aspects