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Title: Subcellular distribution of potassium in striated muscles

Abstract

Microanalytical experiments have been performed to answer the question whether the main cellular cation, K+, follows the water distribution in the striated muscle cell or whether K+ follows the distribution of negative fixed charges (beta- and gamma-carboxyl groups of aspartic and glutamic acid residues). Subcellular localization of K and/or of the K surrogates Rb, Cs, and Tl has been investigated by the following methods: Chemical precipitation of K with tetraphenylborate. Autoradiography of alkali-metals and Tl in air-dried and frozen-hydrated preparations. TEM visualization of electron dense Cs and Tl in sections of freeze-dried and plastic embedded muscle. X-ray microanalysis of air-dried myofibrils and muscle cryosections. The experiments consistently show that K, Rb, Cs, and Tl do not follow the water distribution but are mainly accumulated in the A band, especially in the marginal regions, and at Z lines. The same sites preferentially accumulate Cs or uranyl cations when sections of freeze-dried, embedded muscle are exposed to these electron microscopic stains. It is concluded that the detected uneven distribution of K, Rb, Cs, and Tl in muscle is neither a freeze-drying artifact nor an embedding artifact and may result from a weak ion binding to the beta- and gamma-carboxyl groups of cellularmore » proteins.« less

Authors:
Publication Date:
Research Org.:
Universitaet des Saarlandes, Homburg/Saar, Germany
OSTI Identifier:
6001114
Resource Type:
Journal Article
Journal Name:
Scanning Electron Microsc.; (United States)
Additional Journal Information:
Journal Name: Scanning Electron Microsc.; (United States)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; CESIUM; TISSUE DISTRIBUTION; MUSCLES; AUTORADIOGRAPHY; ELECTRON MICROPROBE ANALYSIS; POTASSIUM; RUBIDIUM; THALLIUM; BEES; BIOLOGICAL ACCUMULATION; COMPARATIVE EVALUATIONS; WATER; ALKALI METALS; ANIMALS; ARTHROPODS; CHEMICAL ANALYSIS; DISTRIBUTION; ELEMENTS; HYDROGEN COMPOUNDS; HYMENOPTERA; INSECTS; INVERTEBRATES; METALS; MICROANALYSIS; OXYGEN COMPOUNDS; 550201* - Biochemistry- Tracer Techniques

Citation Formats

Edelmann, L. Subcellular distribution of potassium in striated muscles. United States: N. p., 1984. Web.
Edelmann, L. Subcellular distribution of potassium in striated muscles. United States.
Edelmann, L. 1984. "Subcellular distribution of potassium in striated muscles". United States.
@article{osti_6001114,
title = {Subcellular distribution of potassium in striated muscles},
author = {Edelmann, L},
abstractNote = {Microanalytical experiments have been performed to answer the question whether the main cellular cation, K+, follows the water distribution in the striated muscle cell or whether K+ follows the distribution of negative fixed charges (beta- and gamma-carboxyl groups of aspartic and glutamic acid residues). Subcellular localization of K and/or of the K surrogates Rb, Cs, and Tl has been investigated by the following methods: Chemical precipitation of K with tetraphenylborate. Autoradiography of alkali-metals and Tl in air-dried and frozen-hydrated preparations. TEM visualization of electron dense Cs and Tl in sections of freeze-dried and plastic embedded muscle. X-ray microanalysis of air-dried myofibrils and muscle cryosections. The experiments consistently show that K, Rb, Cs, and Tl do not follow the water distribution but are mainly accumulated in the A band, especially in the marginal regions, and at Z lines. The same sites preferentially accumulate Cs or uranyl cations when sections of freeze-dried, embedded muscle are exposed to these electron microscopic stains. It is concluded that the detected uneven distribution of K, Rb, Cs, and Tl in muscle is neither a freeze-drying artifact nor an embedding artifact and may result from a weak ion binding to the beta- and gamma-carboxyl groups of cellular proteins.},
doi = {},
url = {https://www.osti.gov/biblio/6001114}, journal = {Scanning Electron Microsc.; (United States)},
number = ,
volume = ,
place = {United States},
year = {Sun Jan 01 00:00:00 EST 1984},
month = {Sun Jan 01 00:00:00 EST 1984}
}