Inactivation of Coxiella burnetti by gamma irradiation
Technical Report
·
OSTI ID:5999767
The gamma radiation inactivation kinetics for Coxiella burnetii at - 79 C were exponential. The radiation dose needed to reduce the number of infective C. burnetii by 90% varied from 0-64 to 1.2 kGy depending on the phase of hte micro-organism, purity of the culture and composition of suspending menstruum. The viability of preparations containing C. burnetti was completely abolished by 10 kGy without diminishing antigenicity or ability to elicit a protective immune response in vaccinated mice. Immunocytochemical examinations using monoclonal antibodies and electron microscopy demonstrated that radiation doses of 20 kGy did not alter cell-wall morphology or cell-surface antigenic epitopes.
- Research Organization:
- Army Medical Research Inst. of Infectious Diseases, Fort Detrick, MD (USA)
- OSTI ID:
- 5999767
- Report Number(s):
- AD-A-227218/5/XAB
- Resource Relation:
- Other Information: Pub. in Jnl. of General Microbiology, Vol. 135, 3263-3270(1989)
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
60 APPLIED LIFE SCIENCES
RICKETTSIAE
BIOLOGICAL RADIATION EFFECTS
RICKETTSIAL DISEASES
VACCINES
ANTIGENS
CELL MEMBRANES
ELECTRON MICROSCOPY
GAMMA RADIATION
IMMUNITY
MICE
MONOCLONAL ANTIBODIES
RADIATION DOSES
ANIMALS
ANTIBODIES
BIOLOGICAL EFFECTS
CELL CONSTITUENTS
DISEASES
DOSES
ELECTROMAGNETIC RADIATION
INFECTIOUS DISEASES
IONIZING RADIATIONS
MAMMALS
MEMBRANES
MICROORGANISMS
MICROSCOPY
RADIATION EFFECTS
RADIATIONS
RODENTS
VERTEBRATES
553003* - Agriculture & Food Technology- Pest & Disease Control- (1987-)
RICKETTSIAE
BIOLOGICAL RADIATION EFFECTS
RICKETTSIAL DISEASES
VACCINES
ANTIGENS
CELL MEMBRANES
ELECTRON MICROSCOPY
GAMMA RADIATION
IMMUNITY
MICE
MONOCLONAL ANTIBODIES
RADIATION DOSES
ANIMALS
ANTIBODIES
BIOLOGICAL EFFECTS
CELL CONSTITUENTS
DISEASES
DOSES
ELECTROMAGNETIC RADIATION
INFECTIOUS DISEASES
IONIZING RADIATIONS
MAMMALS
MEMBRANES
MICROORGANISMS
MICROSCOPY
RADIATION EFFECTS
RADIATIONS
RODENTS
VERTEBRATES
553003* - Agriculture & Food Technology- Pest & Disease Control- (1987-)