Sequence comparison in the crossover region of an oncogenic avian retrovirus recombinant and its nononcogenic parent: Genetic regions that control growth rate and oncogenic potential

Journal Article · Mon Nov 01 00:00:00 EST 1982 · Mol. Cell. Biol.; (United States)

DOI:https://doi.org/10.1128/MCB.2.11.1331· OSTI ID:5996595

Tsichlis, P N; Donehower, L; Hager, G; Zeller, N; Malavarca, R; Astrin, S; Skalka, A M

NTRE is an avian retrovirus recombinant of the endogeneous nononcogenic Rous-associated virus-0 (RAV-0) and the oncogenic, exogeneous, transformation-defective (td) Prague strain of Rous sarcoma virus B (td-PrRSV-B). Oligonucleotide mapping had shown that the recombinant virus is indistinguishable from its RAV-0 parent except for the 3'-end sequences, which were derived from td-PrRSV-B. However, the virus exhibits properties which are typical of an exogenous virus: it grows to high titers in tissue culture, and it is oncogenic in vivo. To accurately define the genetic region responsible for these properties, the authors determined the nucleotide sequences of the recombinant and its RAV-0 parent by using molecular clones of their DNA. These were compared with sequences already available for PrRSV-C, a virus closely related to the exogenous parent td-PrRSV-B. The results suggested that the crossover event which generated NTRE 7 took place in a region -501 to -401 nucleotides from the 3' end of the td-PrRSV parental genome and that sequences to the right of the recombination region were responsible for its growth properties and oncogenic potential. Since the exogenous-virus-specific sequences are expected to be missing from transformation-defective mutants of the Schmidt-Ruppin strain of RSV, which, like other exogeneous viruses, grow to high tiers in tissue culture and are oncogenic in vivo, the authors concluded that the growth properties and oncogenic potential of the exogeneous viruses are determined by sequences in the U3 region of the long terminal repeat. However, the authors propose that the exogeneous-virus-specific region may play a role in determining the oncogenic spectrum of a given oncogenic virus.

• Research Organization: Lab. of Tumor Virus Genetics, National Cancer Institute, Bethesda, MD 20205
• OSTI ID: 5996595
• Journal Information: Mol. Cell. Biol.; (United States), Vol. 2:11
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ONCOGENES
DNA SEQUENCING
ONCOGENIC VIRUSES
DNA-CLONING
GENETIC MAPPING
BIRDS
CELL PROLIFERATION
DEFECTS
GENETIC CONTROL
IN VIVO
MUTANTS
OLIGONUCLEOTIDES
ONCOGENIC TRANSFORMATIONS
RECOMBINANT DNA
TISSUE CULTURES
ANIMALS
CELL TRANSFORMATIONS
CLONING
CONTROL
DNA
GENES
MAPPING
MICROORGANISMS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PARASITES
PEST CONTROL
STRUCTURAL CHEMICAL ANALYSIS
VERTEBRATES
VIRUSES
550400* - Genetics
550200 - Biochemistry