Separations of compounds of biological and environmental interest by micellar electrokinetic capillary chromatography

Conference · Sat Oct 01 00:00:00 EDT 1988 · Sep. Sci. Technol.; (United States)

OSTI ID:5995775

Balchunas, A T; Swaile, D F; Powell, A C; Sepaniak, M J

Important criteria for the effective separation of compounds of biological or environmental interest by micellar electrokinetic capillary chromatography are discussed. Efficiencies of approximately 100,000 plates/meter are achieved in the separations of samples of derivatized amines, aflatoxins, and hydroxy aromatic compounds. Laser fluorometric detection is shown to be capable of detecting subpicogram injected quantities. Organic solvents such as 2-propanol and acetonitrile are added to the aqueous mobile phases normally used to improve the separation of hydrophobic compounds, impart different selectivities, and provide a means for gradient programming. Column diameter is found to influence efficiency, analysis time, and detection.

• Research Organization: Univ. of Tennessee, Knoxville (USA)
• OSTI ID: 5995775
• Report Number(s): CONF-871038-
• Journal Information: Sep. Sci. Technol.; (United States), Vol. 23:12,13; Conference: 5. symposium on separation science and technology for energy applications, Knoxville, TN, USA, 26 Oct 1987
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC
ORGANIC
PHYSICAL AND ANALYTICAL CHEMISTRY
AFLATOXIN
CHROMATOGRAPHY
AMINES
AROMATICS
HYDROXY COMPOUNDS
CAPILLARIES
EFFICIENCY
ELECTROCHEMISTRY
EXPERIMENTAL DATA
MICELLAR SYSTEMS
ORGANIC SOLVENTS
ANTIGENS
BLOOD VESSELS
BODY
CARDIOVASCULAR SYSTEM
CHEMISTRY
DATA
INFORMATION
MATERIALS
NUMERICAL DATA
ORGANIC COMPOUNDS
ORGANS
SEPARATION PROCESSES
SOLVENTS
TOXIC MATERIALS
TOXINS
400105* - Separation Procedures
400400 - Electrochemistry