Effect of sodium selenite on the ciliary activity, adenosine triphosphate, and protein synthesis in mouse trachea organ cultures

Journal Article · Sun Jan 01 00:00:00 EST 1984 · J. Toxicol. Environ. Health; (United States)

DOI:https://doi.org/10.1080/15287398409530546· OSTI ID:5994936

Lag, M; Paulsen, G; Jonsen, J

Trachea from albino mice were cut transversely into nearly identical rings and incubated in medium 199 with Hanks salts and HEPES buffer at 37/sup 0/C. Sodium selenite at 0.5-5 mM depressed the ciliary activity. With 1 and 5 mM sodium selenite, a 50% reduction in the activity index was observed after approximately 5 and 1.5 h, respectively. The ATP content in trachea rings was reduced with 0.05-5 mM sodium selenite, and increasing concentrations gave decreasing amount of ATP after incubation for 4 and 21 h. The rate of protein synthesis as determined by incorporation of radioactive leucine was reduced with 0.5 and 2 mM sodium selenite. The synthesis was reduced quickly by 2 mM sodium selenite, which gave a 30% reduction after incubation for 1 h. 16 references, 2 figures, 3 tables.

• Research Organization: Univ. of Oslo, Blindern, Norway
• OSTI ID: 5994936
• Journal Information: J. Toxicol. Environ. Health; (United States), Vol. 13:4-6
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ATP
INHIBITION
PROTEINS
BIOSYNTHESIS
SELENIUM
TOXICITY
BIOLOGICAL INDICATORS
EXPERIMENTAL DATA
LABELLING
LEUCINE
MICE
TRACHEA
AMINO ACIDS
ANIMALS
CARBOXYLIC ACIDS
DATA
ELEMENTS
INFORMATION
MAMMALS
NUCLEOTIDES
NUMERICAL DATA
ORGANIC ACIDS
ORGANIC COMPOUNDS
RESPIRATORY SYSTEM
RODENTS
SEMIMETALS
SYNTHESIS
VERTEBRATES
560305* - Chemicals Metabolism & Toxicology- Vertebrates- (-1987)