Improvement of the periodate-borohydride surface-labeling method for human blood platelets
Abstract
The periodate/sodium boro(/sup 3/H)hydride ((/sup 3/H)-NaBH4) method is extensively used for the specific labeling of cell surface glycoproteins. Reduction with tritiated borohydride is also used in other surface-labeling techniques, the neuraminidase/galactose oxidase/(/sup 3/H)-NaBH4 method (specific for terminal galactose and N-acetyl-galactosamine residues) and the pyridoxal phosphate/(/sup 3/H)-NaBH4 method (specific for protein). By modification of the reaction conditions during the periodate-oxidation and borohydride-reduction, the ratio of the incorporated to the total added radioactivity could be increased by a factor of 50, while the specific activity of the labeled material was twice as high as in the original method. Alternatively, by another modification, the specific activity of the labeled material could be increased about 10-fold. The influence of the most important parameters was investigated in detail. Sodium dodecyl sulfate gel electrophoresis and fluorography demonstrate that the labeling pattern of the membrane glycoproteins is the same as with the conventional method.
- Authors:
- Publication Date:
- Research Org.:
- Theodor Kocher Institute, University of Berne, Switzerland
- OSTI Identifier:
- 5994746
- Resource Type:
- Journal Article
- Journal Name:
- Thromb. Res.; (United States)
- Additional Journal Information:
- Journal Volume: 29:1
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 38 RADIATION CHEMISTRY, RADIOCHEMISTRY, AND NUCLEAR CHEMISTRY; BLOOD PLATELETS; LABELLING; GLUCOPROTEINS; BOROHYDRIDES; CHEMICAL PREPARATION; LABELLED COMPOUNDS; PERIODATES; SODIUM COMPOUNDS; TRITIUM COMPOUNDS; ALKALI METAL COMPOUNDS; BIOLOGICAL MATERIALS; BLOOD; BLOOD CELLS; BODY FLUIDS; BORON COMPOUNDS; CARBOHYDRATES; HALOGEN COMPOUNDS; HYDROGEN COMPOUNDS; IODINE COMPOUNDS; MATERIALS; ORGANIC COMPOUNDS; OXYGEN COMPOUNDS; PROTEINS; SACCHARIDES; SYNTHESIS; 400702* - Radiochemistry & Nuclear Chemistry- Properties of Radioactive Materials
Citation Formats
Steiner, B, Clemetson, K J, and Luescher, E F. Improvement of the periodate-borohydride surface-labeling method for human blood platelets. United States: N. p., 1983.
Web. doi:10.1016/0049-3848(83)90124-X.
Steiner, B, Clemetson, K J, & Luescher, E F. Improvement of the periodate-borohydride surface-labeling method for human blood platelets. United States. https://doi.org/10.1016/0049-3848(83)90124-X
Steiner, B, Clemetson, K J, and Luescher, E F. 1983.
"Improvement of the periodate-borohydride surface-labeling method for human blood platelets". United States. https://doi.org/10.1016/0049-3848(83)90124-X.
@article{osti_5994746,
title = {Improvement of the periodate-borohydride surface-labeling method for human blood platelets},
author = {Steiner, B and Clemetson, K J and Luescher, E F},
abstractNote = {The periodate/sodium boro(/sup 3/H)hydride ((/sup 3/H)-NaBH4) method is extensively used for the specific labeling of cell surface glycoproteins. Reduction with tritiated borohydride is also used in other surface-labeling techniques, the neuraminidase/galactose oxidase/(/sup 3/H)-NaBH4 method (specific for terminal galactose and N-acetyl-galactosamine residues) and the pyridoxal phosphate/(/sup 3/H)-NaBH4 method (specific for protein). By modification of the reaction conditions during the periodate-oxidation and borohydride-reduction, the ratio of the incorporated to the total added radioactivity could be increased by a factor of 50, while the specific activity of the labeled material was twice as high as in the original method. Alternatively, by another modification, the specific activity of the labeled material could be increased about 10-fold. The influence of the most important parameters was investigated in detail. Sodium dodecyl sulfate gel electrophoresis and fluorography demonstrate that the labeling pattern of the membrane glycoproteins is the same as with the conventional method.},
doi = {10.1016/0049-3848(83)90124-X},
url = {https://www.osti.gov/biblio/5994746},
journal = {Thromb. Res.; (United States)},
number = ,
volume = 29:1,
place = {United States},
year = {Sat Jan 01 00:00:00 EST 1983},
month = {Sat Jan 01 00:00:00 EST 1983}
}