Major surface antigen, P30, of Toxoplasma gondii is anchored by a glycolipid
P30, the major surface antigen of the parasitic protozoan Toxoplasma gondii, can be specifically labeled with (/sup 3/H)palmitic acid and with myo-(2-/sup 3/H)inositol. The fatty acid label can be released by treatment of P30 with phosphatidylinositol-specific phospholipase C (PI-PLC). Such treatment exposes an immunological cross-reacting determinant first described on Trypanosoma brucei variant surface glycoprotein. PI-PLC cleavage of intact parasites metabolically labeled with (/sup 35/S)methionine results in the release of intact P30 polypeptide in a form which migrates faster in polyacrylamide gel electrophoresis. These results argue that P30 is anchored by a glycolipid. Results from thin layer chromatography analysis of purified (/sup 3/H) palmitate-labeled P30 treated with PI-PLC, together with susceptibility to mild alkali hydrolysis and to cleavage with phospholipase A2, suggest that the glycolipid anchor of T. gondii P30 includes a 1,2-diacylglycerol moiety.
- Research Organization:
- Stanford Univ. School of Medicine, CA (USA)
- OSTI ID:
- 5991470
- Journal Information:
- J. Biol. Chem.; (United States), Vol. 264:10
- Country of Publication:
- United States
- Language:
- English
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ANTIGENS
LABELLING
GLYCOLIPIDS
BIOLOGICAL FUNCTIONS
LIPASE
BIOCHEMICAL REACTION KINETICS
AMINO ACID SEQUENCE
ELECTROPHORESIS
HEXADECANOIC ACID
INOSITOL
METHIONINE
PHOSPHOLIPIDS
PROTOZOA
THIN-LAYER CHROMATOGRAPHY
TRITIUM COMPOUNDS
AMINO ACIDS
ANIMALS
CARBOHYDRATES
CARBOXYLESTERASES
CARBOXYLIC ACIDS
CHROMATOGRAPHY
DRUGS
ENZYMES
ESTERASES
ESTERS
FUNCTIONS
HYDROLASES
INOSITOLS
INVERTEBRATES
KINETICS
LABELLED COMPOUNDS
LIPIDS
LIPOTROPIC FACTORS
MICROORGANISMS
MOLECULAR STRUCTURE
MONOCARBOXYLIC ACIDS
MONOSACCHARIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANIC SULFUR COMPOUNDS
REACTION KINETICS
SACCHARIDES
SEPARATION PROCESSES
550201* - Biochemistry- Tracer Techniques