Calcification of in vitro developed hypertrophic cartilage

Journal Article · Sat Apr 01 00:00:00 EST 1989 · Dev. Biol.; (United States)

DOI:https://doi.org/10.1016/0012-1606(89)90240-6· OSTI ID:5991422

Tacchetti, C; Quarto, R; Campanile, G; Cancedda, R

We have recently reported that dedifferentiated cells derived from stage 28-30 chick embryo tibiae, when transferred in suspension culture in the presence of ascorbic acid, develop in a tissue closely resembling hypertrophic cartilage. Ultrastructural examination of this in vitro formed cartilage showed numerous matrix vesicles associated with the extracellular matrix. In the present article we report that the in vitro developed hypertrophic cartilage undergoes calcification. We indicate a correlation between the levels of alkaline phosphatase activity and calcium deposition at different times of development. Following the transfer of cells into suspension culture and an initial lag phase, the level of alkaline phosphatase activity rapidly increased. In most experiments the maximum of activity was reached after 5 days of culture. When alkaline phosphatase activity and /sup 45/Ca deposition were measured in the same experiment, we observed that the increase in alkaline phosphatase preceded the deposition of nonwashable calcium deposits in the cartilage.

• Research Organization: Istituto Nazionale per la Ricerca sul Cancro, Genova (Italy)
• OSTI ID: 5991422
• Journal Information: Dev. Biol.; (United States), Vol. 132:2
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ALKALINE PHOSPHATASE
ENZYME ACTIVITY
CARTILAGE
CALCINOSIS
CHICKENS
ONTOGENESIS
ASCORBIC ACID
CALCIUM
CALCIUM 45
CELL CULTURES
EMBRYOS
FLUORESCENCE
IN VITRO
STAINS
TRACER TECHNIQUES
ALKALINE EARTH ISOTOPES
ALKALINE EARTH METALS
ANIMAL TISSUES
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIRDS
BODY
CALCIUM ISOTOPES
CONNECTIVE TISSUE
DAYS LIVING RADIOISOTOPES
ELEMENTS
ENZYMES
ESTERASES
EVEN-ODD NUCLEI
FOWL
HYDROLASES
INTERMEDIATE MASS NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
LUMINESCENCE
METALS
NUCLEI
PATHOLOGICAL CHANGES
PHOSPHATASES
RADIOISOTOPES
TISSUES
VERTEBRATES
VITAMINS
550201* - Biochemistry- Tracer Techniques