Synthesis and functioning of the colicin E1 lysis protein: Comparison with the colicin A lysis protein

Journal Article · Tue Jan 01 00:00:00 EST 1991 · Journal of Bacteriology; (USA)

OSTI ID:5989354

Cavard, D ^[1]

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1. Centre de Biochimie et de Biologie Moleculaire du C.N.R.S., Marseille (France)

The colicin E1 lysis protein, CelA, was identified as a 3-kDa protein in induced cells of Escherichia coli K-12 carrying pColE1 by pulse-chase labeling with either ({sup 35}S)cysteine or ({sup 3}H)lysine. This 3-kDa protein was acylated, as shown by (2-{sup 3}H)glycerol labeling, and seemed to correspond to the mature CelA protein. The rate of modification and processing of CelA was different from that observed for Cal, the colicin A lysis protein. In contrast to Cal, no intermediate form was detected for CelA, no signal peptide accumulated, and no modified precursor form was observed after globomycin treatment. Thus, the rate of synthesis would not be specific to lysis proteins. Solubilization in sodium dodecyl sulfate of the mature forms of both CelA and Cal varied similarly at the time of colicin release, indicating a change in lysis protein structure. This particular property would play a role in the mechanism of colicin export. The accumulation of the signal peptide seems to be a factor determining the toxicity of the lysis proteins since CelA provoked less cell damage than Cal. Quasi-lysis and killing due to CelA were higher in degP mutants than in wild-type cells. They were minimal in pldA mutants.

• OSTI ID: 5989354
• Journal Information: Journal of Bacteriology; (USA), Vol. 173:1; ISSN 0021-9193
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
PROTEINS
BIOSYNTHESIS
COMPARATIVE EVALUATIONS
CYSTEINE
ESCHERICHIA COLI
GLYCEROL
LYSINE
LYSIS
MOLECULAR WEIGHT
MUTANTS
PLASMIDS
PROTEIN STRUCTURE
SULFUR 35
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ALCOHOLS
AMINO ACIDS
BACTERIA
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBOXYLIC ACIDS
CELL CONSTITUENTS
DAYS LIVING RADIOISOTOPES
EVEN-ODD NUCLEI
HYDROGEN COMPOUNDS
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MICROORGANISMS
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
RADIOISOTOPES
SULFUR ISOTOPES
SYNTHESIS
THIOLS
550201* - Biochemistry- Tracer Techniques