Production and characterization of monoclonal antibodies against rat platelet GPIIb/IIIa

Journal Article · Sat Sep 15 00:00:00 EDT 1990 · Thrombosis Research; (USA)

DOI:https://doi.org/10.1016/0049-3848(90)90118-V· OSTI ID:5989006

Miyazaki, H; Tamura, S; Sudo, T; Suzuki, T ^[1]

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1. Kirin Brewery Co., Ltd., Gunma (Japan)

Four murine monoclonal antibodies against rat platelets were produced by fusion of spleen cells from mice intravenously immunized with whole rat platelets. All four antibodies immunoprecipitated two major platelet membrane proteins with apparent molecular weights of 130,000 and 82,000 (nonreduced) and of 120,000 and 98,000 (reduced), which were structurally analogous to human glycoprotein (GP) IIb/IIIa, i.e. rat GPIIb/IIIa. Two of four antibodies, named P9 and P55, strongly inhibited adenosine diphosphate (ADP)-induced aggregation of washed rat platelets and caused approximately 50% inhibition of human fibrinogen binding to ADP-stimulated rat platelets, suggesting that rat GPIIb/IIIa serves as a fibrinogen receptor in ADP-induced aggregation. In contrast, two other antibodies, named P14 and P34, themselves caused aggregation of rat platelets in platelet-rich plasma (PRP) and the secretion of 14C-serotonin from 14C-serotonin-labeled PRP. These results indicate that rat GPIIb/IIIa plays an important role in platelet aggregation.

• OSTI ID: 5989006
• Journal Information: Thrombosis Research; (USA), Vol. 59:6; ISSN 0049-3848
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
BLOOD PLATELETS
BLOOD COAGULATION
MEMBRANE PROTEINS
ENZYME IMMUNOASSAY
MONOCLONAL ANTIBODIES
CHEMICAL COMPOSITION
ADP
CARBON 14 COMPOUNDS
FIBRINOGEN
INHIBITION
MAN
MICE
RATS
RECEPTORS
SEROTONIN
SPLEEN CELLS
AMINES
ANIMAL CELLS
ANIMALS
ANTIBODIES
AROMATICS
AUTONOMIC NERVOUS SYSTEM AGENTS
AZAARENES
AZOLES
BIOASSAY
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BLOOD COAGULATION FACTORS
BODY FLUIDS
COAGULANTS
DRUGS
GLOBULINS
HEMATOLOGIC AGENTS
HEMOSTATICS
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
IMMUNOASSAY
INDOLES
LABELLED COMPOUNDS
MAMMALS
MATERIALS
NEUROREGULATORS
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PRIMATES
PROTEINS
PYRROLES
RADIOPROTECTIVE SUBSTANCES
RODENTS
SOMATIC CELLS
SYMPATHOMIMETICS
TRYPTAMINES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques