Astrocyte cultures derived from human brain tissue express angiotensinogen mRNA

Journal Article · Wed Aug 01 00:00:00 EDT 1990 · Proceedings of the National Academy of Sciences of the United States of America; (USA)

DOI:https://doi.org/10.1073/pnas.87.15.5720· OSTI ID:5988512

Milsted, A; Barna, B P; Ransohoff, R M; Brosnihan, K B; Ferrario, C M ^[1]

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1. Cleveland Clinic Foundation, OH (USA)

The authors have identified human cultured cell lines that are useful for studying angiotensinogen gene expression and its regulation in the central nervous system. A model cell system of human central nervous system origin expressing angiotensinogen has not previously been available. Expression of angiotensinogen mRNA appears to be a basal property of noninduced human astrocytes, since astrocytic cell lines derived from human glioblastomas or nonneoplastic human brain tissue invariably produced angiotensinogen mRNA. In situ hybridization histochemistry revealed that angiotensinogen mRNA production was not limited to a subpopulation of astrocytes because >99% of cells in these cultures contained angiotensinogen mRNA. These cell lines will be useful in studies of the molecular mechanisms controlling angiotensin synthesis and the role of biologically active angiotensin in the human brain by allowing the authors to examine regulation of expression of the renin-angiotensin system in human astrocyte cultures.

• OSTI ID: 5988512
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (USA), Vol. 87:15; ISSN 0027-8424
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
ANGIOTENSIN
GENE REGULATION
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AUTORADIOGRAPHY
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CYTOCHEMISTRY
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ANIMALS
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BIOCHEMISTRY
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CENTRAL NERVOUS SYSTEM
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DRUGS
ENZYMES
GLOBULINS
HYBRIDIZATION
HYDROLASES
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NONSPECIFIC PEPTIDASES
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANS
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PRIMATES
PROTEINS
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550201* - Biochemistry- Tracer Techniques