Cysteamine and prostaglandin F2 beta stimulate rat gastric mucin release

Journal Article · Tue Feb 01 00:00:00 EST 1983 · Gastroenterology; (United States)

OSTI ID:5983750

Lamont, J T; Ventola, A S; Maull, E A; Szabo, S

Gastric mucin glycoproteins form an adherent gel over the surface epithelium that is thought to protect the stomach against chemical and physical damage. The purpose of this study was to measure the release of mucin glycoproteins from rat stomach after treatment with cysteamine and prostaglandin F2 beta, two structurally unrelated drugs that have been shown to protect the stomach against the noxious effects of alcohol and other damaging agents. Gastric mucin was separated into soluble (washout) and insoluble (adherent) phases before colorimetric quantitation of total mucin, protein-bound hexose, and sialic acid. Cysteamine produced a dose-dependent increase in release of soluble and gel mucin. Prostaglandin F2 beta caused a dose-dependent release of hexose-containing mucin but had no effect on sialic acid-containing glycoproteins. Sepharose 4B chromatography of both the soluble and adherent mucus revealed that greater than 90% was a high molecular weight glycoprotein fraction. N-Ethylmaleimide, a known inhibitor of cytoprotection by cysteamine, had no effect on mucin secretion. Similarly, indomethacin inhibited mucin secretion by cysteamine but did not significantly influence cytoprotection. Thus the secretion of mucin by cytoprotective agents is unlikely by itself to explain the ability of the stomach to resist chemical or physical damage.

• Research Organization: Evans Memorial Department of Clinical Research, University Hospital, Boston, Massachusetts
• OSTI ID: 5983750
• Journal Information: Gastroenterology; (United States), Vol. 84:2
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
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GLUCOPROTEINS
SECRETION
MEA
BIOLOGICAL EFFECTS
PROSTAGLANDINS
BIOCHEMISTRY
DOSE-RESPONSE RELATIONSHIPS
EPITHELIUM
MUCOUS MEMBRANES
RATS
STIMULATION
STOMACH
AMINES
ANIMAL TISSUES
ANIMALS
BODY
CARBOHYDRATES
CHEMISTRY
DIGESTIVE SYSTEM
DRUGS
GASTROINTESTINAL TRACT
MAMMALS
MEMBRANES
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANS
PROTEINS
RADIOPROTECTIVE SUBSTANCES
RODENTS
SACCHARIDES
THIOLS
TISSUES
VERTEBRATES
560305* - Chemicals Metabolism & Toxicology- Vertebrates- (-1987)
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