Genomic localization, sequence analysis, and transcription of the putative human cytomegalovirus DNA polymerase gene

Journal Article · Thu Jan 01 00:00:00 EST 1987 · J. Virol.; (United States)

OSTI ID:5982803

Heilbronn, T; Jahn, G; Buerkle, A; Freese, U K; Fleckenstein, B; Zur Hausen, H

The human cytomegalovirus (HCMV)-induced DNA polymerase has been well characterized biochemically and functionally, but its genomic location has not yet been assigned. To identify the coding sequence, cross-hybridization with the herpes simplex virus type 1 (HSV-1) polymerase gene was used, as suggested by the close similarity of the herpes group virus-induced DNA polymerases to the HCMV DNA polymerase. A cosmid and plasmid library of the entire HCMV genome was screened with the BamHI Q fragment of HSF-1 at different stringency conditions. One PstI-HincII restriction fragment of 850 base pairs mapping within the EcoRI M fragment of HCMV cross-hybridized at T/sub m/ - 25/degrees/C. Sequence analysis revealed one open reading frame spanning the entire sequence. The amino acid sequence showed a highly conserved domain of 133 amino acids shared with the HSV and putative Esptein-Barr virus polymerase sequences. This domain maps within the C-terminal part of the HSV polymerase gene, which has been suggested to contain part of the catalytic center of the enzyme. Transcription analysis revealed one 5.4-kilobase early transcript in the sense orientation with respect to the open reading frame identified. This transcript appears to code for the 140-kilodalton HCMV polymerase protein.

• Research Organization: Universitaet Erlangen-Nuernbert (Germany, F.R.)
• OSTI ID: 5982803
• Journal Information: J. Virol.; (United States), Vol. 61:1
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
DNA POLYMERASES
GENETIC MAPPING
VIRUSES
HYBRIDIZATION
DNA SEQUENCING
HERPES SIMPLEX
NUCLEOTIDES
PHOSPHORUS 32
TRANSCRIPTION
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
DAYS LIVING RADIOISOTOPES
DISEASES
ENZYMES
INFECTIOUS DISEASES
ISOTOPES
LIGHT NUCLEI
MAPPING
MICROORGANISMS
NUCLEI
NUCLEOTIDYLTRANSFERASES
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PARASITES
PHOSPHORUS ISOTOPES
PHOSPHORUS-GROUP TRANSFERASES
POLYMERASES
RADIOISOTOPES
SKIN DISEASES
STRUCTURAL CHEMICAL ANALYSIS
TRANSFERASES
VIRAL DISEASES
550201* - Biochemistry- Tracer Techniques