Application of continuous substrate feeding to the ABE fermentation: Relief of product inhibition using extraction, perstraction, stripping, and pervaporation
- Massey Univ., Palmerston North (New Zealand)
The technique of continuous substrate feeding has been applied to the batch fermentation process using freely suspended cells, for ABE (acetone-butanol-ethanol) production. To avoid the product inhibition which normally restricts ABE production to less than 20 g/L and sugar utilization to 60 g/L, a product removal technique has been integrated into the fermentation process. The techniques investigated were liquid-liquid extraction, perstraction, gas-stripping, and pervaporation. By using a substrate of whey permeate, the reactor productivity has been improved over that observed in a traditional batch fermentation, while equivalent lactose utilization and ABE production values of 180 g and 69 g, respectively, have been achieved in a 1-L culture volume. 17 refs., 14 figs., 5 tabs.
- Sponsoring Organization:
- USDOE
- OSTI ID:
- 598194
- Journal Information:
- Biotechnology Progress, Vol. 8, Issue 5; Other Information: PBD: Sep-Oct 1992
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
55 BIOLOGY AND MEDICINE
BASIC STUDIES
ACETONE
ETHANOL
EXTRACTION
FERMENTATION
INHIBITION
LACTOSE
PRODUCTION
PRODUCTIVITY
VOLUME
WHEY
SUBSTRATES
BATCH CULTURE
BUTANOLS
OPTIMIZATION
ANAEROBIC DIGESTION
CLOSTRIDIUM ACETOBUTYLICUM
CHEMICAL FEEDSTOCKS
LIQUID FUELS
CONTINUOUS CULTURE
CONCENTRATION RATIO