skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Chlororespiration: An adaptation to nitrogen deficiency in Chlamydomonas reinhardtii

Abstract

When grown under nitrogen limitation, pronounced chlororespiratory activity develops together with an altered composition of thylakoid membranes in Chlamydomonas reinhardtii. Relative to control cultures, the flash-inhibited, chlororespiration-dependent O{sub 2} consumption signal increases 10-fold. Also augmented is the light-sensitive respiratory activity responsible of the Kok effect, reflecting competitive inhibition of chlororespiratory electron transport by photosystem I. Fluoresscence measurements show that the thylakoid plastoquinone pool is extensively reduced in dark-adapted, N-limited cells. Thylakoids of N-limited cells have reduced amounts of cytochrome b{sub 6}, cytochrome f, and light-harvesting complexes. However, thylakoid-bound NADH-PQ oxidoreductase, with major subunits of 51 kDa and 17 kDa, is increased 7-fold and two novel cytochromes of 34 and 12.5 kDa are highly abundant. Thus, components of photosynthetic and chlororespiratory electron transport pathways are differentially regulated by N availability.

Authors:
;  [1]
  1. Univ. of Georgia, Athens (United States)
Publication Date:
OSTI Identifier:
5976389
DOE Contract Number:  
FG09-84ER13188
Resource Type:
Journal Article
Journal Name:
Proceedings of the National Academy of Sciences of the United States of America; (United States)
Additional Journal Information:
Journal Volume: 88:11; Journal ID: ISSN 0027-8424
Country of Publication:
United States
Language:
English
Subject:
14 SOLAR ENERGY; CHLAMYDOMONAS; PHOTOSYNTHESIS; CHLOROPLASTS; RESPIRATION; OXIDOREDUCTASES; ENZYME ACTIVITY; CYTOCHROME OXIDASE; ELECTRON TRANSFER; FLUORESCENCE; NITROGEN; NUTRITIONAL DEFICIENCY; THYLAKOID MEMBRANE PROTEINS; ALGAE; CELL CONSTITUENTS; CHEMICAL REACTIONS; CHLOROPHYCOTA; ELEMENTS; ENZYMES; HAEM DEHYDROGENASES; LUMINESCENCE; MEMBRANE PROTEINS; MICROORGANISMS; NONMETALS; ORGANIC COMPOUNDS; PHOTOCHEMICAL REACTIONS; PLANTS; PROTEINS; SYNTHESIS; UNICELLULAR ALGAE; 140505* - Solar Energy Conversion- Photochemical, Photobiological, & Thermochemical Conversion- (1980-)

Citation Formats

Peltier, G, and Schmidt, G W. Chlororespiration: An adaptation to nitrogen deficiency in Chlamydomonas reinhardtii. United States: N. p., 1991. Web. doi:10.1073/pnas.88.11.4791.
Peltier, G, & Schmidt, G W. Chlororespiration: An adaptation to nitrogen deficiency in Chlamydomonas reinhardtii. United States. https://doi.org/10.1073/pnas.88.11.4791
Peltier, G, and Schmidt, G W. 1991. "Chlororespiration: An adaptation to nitrogen deficiency in Chlamydomonas reinhardtii". United States. https://doi.org/10.1073/pnas.88.11.4791.
@article{osti_5976389,
title = {Chlororespiration: An adaptation to nitrogen deficiency in Chlamydomonas reinhardtii},
author = {Peltier, G and Schmidt, G W},
abstractNote = {When grown under nitrogen limitation, pronounced chlororespiratory activity develops together with an altered composition of thylakoid membranes in Chlamydomonas reinhardtii. Relative to control cultures, the flash-inhibited, chlororespiration-dependent O{sub 2} consumption signal increases 10-fold. Also augmented is the light-sensitive respiratory activity responsible of the Kok effect, reflecting competitive inhibition of chlororespiratory electron transport by photosystem I. Fluoresscence measurements show that the thylakoid plastoquinone pool is extensively reduced in dark-adapted, N-limited cells. Thylakoids of N-limited cells have reduced amounts of cytochrome b{sub 6}, cytochrome f, and light-harvesting complexes. However, thylakoid-bound NADH-PQ oxidoreductase, with major subunits of 51 kDa and 17 kDa, is increased 7-fold and two novel cytochromes of 34 and 12.5 kDa are highly abundant. Thus, components of photosynthetic and chlororespiratory electron transport pathways are differentially regulated by N availability.},
doi = {10.1073/pnas.88.11.4791},
url = {https://www.osti.gov/biblio/5976389}, journal = {Proceedings of the National Academy of Sciences of the United States of America; (United States)},
issn = {0027-8424},
number = ,
volume = 88:11,
place = {United States},
year = {Sat Jun 01 00:00:00 EDT 1991},
month = {Sat Jun 01 00:00:00 EDT 1991}
}