Pre-incubation and low temperatures in quantitative radioreceptor assays
Journal Article
·
· Anal. Lett.; (United States)
The detection limits of drugs in quantitative RRA are primarily determined by their affinities towards the receptor. Yet, the concentration of radiolabeled ligand, necessary for quantification of receptor-bound drug, increases the theoretical detection limit. Therefore the influences of low temperatures and pre-incubation on the detection limit was studied. Analysis of experimental data suggests that when a well-defined incubation procedure is used, pre-incubation and low temperatures will increase sensitivity without loss of accuracy and precision. 6 references, 2 figures.
- Research Organization:
- State Univ. of Groningen, Netherlands
- OSTI ID:
- 5974087
- Journal Information:
- Anal. Lett.; (United States), Vol. 17:B14
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
62 RADIOLOGY AND NUCLEAR MEDICINE
ATROPINE
RADIORECEPTOR ASSAY
IMIDES
PROMETHAZINE
BRAIN
EXPERIMENTAL DATA
INCUBATION
MEAT
RECEPTORS
SAMPLE PREPARATION
SENSITIVITY
TIME DEPENDENCE
TRITIUM COMPOUNDS
ALKALOIDS
ANTIHISTAMINICS
AUTONOMIC NERVOUS SYSTEM AGENTS
BODY
CENTRAL NERVOUS SYSTEM
DATA
DRUGS
FOOD
INFORMATION
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
NERVOUS SYSTEM
NUMERICAL DATA
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PARASYMPATHOLYTICS
TRACER TECHNIQUES
550601* - Medicine- Unsealed Radionuclides in Diagnostics
ATROPINE
RADIORECEPTOR ASSAY
IMIDES
PROMETHAZINE
BRAIN
EXPERIMENTAL DATA
INCUBATION
MEAT
RECEPTORS
SAMPLE PREPARATION
SENSITIVITY
TIME DEPENDENCE
TRITIUM COMPOUNDS
ALKALOIDS
ANTIHISTAMINICS
AUTONOMIC NERVOUS SYSTEM AGENTS
BODY
CENTRAL NERVOUS SYSTEM
DATA
DRUGS
FOOD
INFORMATION
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
NERVOUS SYSTEM
NUMERICAL DATA
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PARASYMPATHOLYTICS
TRACER TECHNIQUES
550601* - Medicine- Unsealed Radionuclides in Diagnostics