Effects of single-dose and fractionated cranial irradiation on rat brain accumulation of methotrexate

Journal Article · Thu Nov 01 00:00:00 EST 1984 · Cancer Res.; (United States)

OSTI ID:5970494

Kamen, B A; Moulder, J E; Kun, L E; Ring, B J; Adams, S M; Fish, B L; Holcenberg, J S

The effects of single-dose and fractionated whole-brain irradiation on brain methotrexate (MTX) has been studied in a rat model. The amount of MTX present in the brain 24 hr after a single i.p. dose (100 mg/kg) was the same whether animals were sham irradiated or given a single dose of 2000 rads 6 or 48 hr prior to the drug (6.9, 8.3, and 6.8 pmol MTX/g, wet weight, respectively). Animals sham irradiated or given 2000 rads in 10 fractions over 11 days and treated with an average dose of 1.2 mg MTX/kg i.p. twice a week for 24 weeks did not differ significantly in their brain MTX concentration (7.9 and 8.3 pmol MTX/g, wet weight, respectively). Chronically MTX-treated animals became folate deficient whether they were irradiated or not (450 and 670 pmol folate/g, wet weight, brain in MTX-treated and control animals). Thus, MTX accumulates in the brain with acute or chronic administration, and this accumulation is not altered by this amount of brain irradiation.

• Research Organization: Medical Coll. of Wisconsin, Milwaukee
• OSTI ID: 5970494
• Journal Information: Cancer Res.; (United States), Vol. 44:11
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BRAIN
BIOLOGICAL RADIATION EFFECTS
METHOTREXATE
UPTAKE
RATS
RADIATION DOSES
BIOLOGICAL MODELS
FOLIC ACID
IRRADIATION
TISSUE DISTRIBUTION
AMINO ACIDS
ANIMALS
ANTIMETABOLITES
AROMATICS
AZAARENES
BIOLOGICAL EFFECTS
BODY
CARBOXYLIC ACIDS
CENTRAL NERVOUS SYSTEM
DISTRIBUTION
DOSES
DRUGS
HEMATINICS
HEMATOLOGIC AGENTS
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
MAMMALS
NERVOUS SYSTEM
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PTERIDINES
RADIATION EFFECTS
RODENTS
VERTEBRATES
VITAMIN B GROUP
VITAMINS
560152* - Radiation Effects on Animals- Animals