Technetium-99m labeling of polymorphonuclear leukocytes: preparation with two different stannous agents
A technique for in vitro labeling of human polymorphonuclear leukocytes with Tc-99m is described. Titration of stannous fluoride and stannous pyrophosphate concentrations for pretinning was performed, and optimal amounts of the stannous agents were added to polymorphonuclear leukocytes efficiency isolated from 100 ml of blood. Labeling with 10-15 mCi Tc-99m resulted, after three washings of cell suspensions, in yield of 1.6-4.8 mCi, corresponding to 20.5-33.5% of added tracer. Cell-bound activity in the final cell suspensions was 92.3% +/- 1.9 of the added dose. Cell function was not impaired by the labeling technique. Sterility and exclusion of bacterial endotoxins in the final cell suspensions were demonstrated. The method may prove of diagnostic value in the isolation, labeling, and reinjection of autologous leukocytes for scintigraphic imaging of acute inflammatory lesions.
- Research Organization:
- Herlev Univ. Hospital, Denmark
- OSTI ID:
- 5970321
- Journal Information:
- J. Nucl. Med.; (United States), Vol. 26:1
- Country of Publication:
- United States
- Language:
- English
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63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
LEUKOCYTES
BIOLOGICAL RADIATION EFFECTS
LABELLING
RADIOPHARMACEUTICALS
CHEMICAL PREPARATION
TECHNETIUM 99
UPTAKE
ISOMERIC NUCLEI
TIN FLUORIDES
TIN PHOSPHATES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
DRUGS
FLUORIDES
FLUORINE COMPOUNDS
HALIDES
HALOGEN COMPOUNDS
HOURS LIVING RADIOISOTOPES
INTERMEDIATE MASS NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
LABELLED COMPOUNDS
MATERIALS
NUCLEI
ODD-EVEN NUCLEI
OXYGEN COMPOUNDS
PHOSPHATES
PHOSPHORUS COMPOUNDS
RADIATION EFFECTS
RADIOISOTOPES
SYNTHESIS
TECHNETIUM ISOTOPES
TIN COMPOUNDS
TIN HALIDES
YEARS LIVING RADIOISOTOPES
400702* - Radiochemistry & Nuclear Chemistry- Properties of Radioactive Materials
560122 - Radiation Effects on Cells- Internal Source- (-1987)