Composition of the cellulase complex of clostridium thermocellum
Journal Article
·
· Biochemistry (Engl. Transl.); (United States)
OSTI ID:5965678
The anaerobic thermophilic cellulolytic bacterium C. thermocellum is considered as a potential organism for the industrial direct bioconversion of cellulose to ethanol. The activities and localization of various cellulolytic enzymes in C. thermocellum are determined here. Preparative methods and determination of enzymes are discussed. Analytical methods are examined. The authors determine that C. thermocellum has all six known cellulolytic enzymes, and that in certain cases up to 50% of the intracellular activity of the indicated enzymes is localized outside the cells.
- Research Organization:
- Institute of the Biochemistry and Physiology of Microorganisms, Academy of Sciences of the USSR, Pushchino
- OSTI ID:
- 5965678
- Journal Information:
- Biochemistry (Engl. Transl.); (United States), Vol. 50:1; Other Information: Translated from Biokhimiya, Vol. 50, No. 1, pp. 109-113, January, 1985
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
09 BIOMASS FUELS
CELLULASE
CHEMICAL COMPOSITION
ENZYMATIC HYDROLYSIS
CLOSTRIDIUM THERMOCELLUM
ETHANOL
PRODUCTION
CELLULOLYTIC ACTIVITY
CELLULOSE
CHEMICAL PREPARATION
FEASIBILITY STUDIES
ALCOHOLS
BACTERIA
CARBOHYDRATES
CHEMICAL REACTIONS
CLOSTRIDIUM
DECOMPOSITION
ENZYME ACTIVITY
ENZYMES
GLYCOSYL HYDROLASES
HYDROLASES
HYDROLYSIS
HYDROXY COMPOUNDS
LYSIS
MICROORGANISMS
O-GLYCOSYL HYDROLASES
ORGANIC COMPOUNDS
POLYSACCHARIDES
SACCHARIDES
SOLVOLYSIS
SYNTHESIS
090222* - Alcohol Fuels- Preparation from Wastes or Biomass- (1976-1989)
140504 - Solar Energy Conversion- Biomass Production & Conversion- (-1989)
CELLULASE
CHEMICAL COMPOSITION
ENZYMATIC HYDROLYSIS
CLOSTRIDIUM THERMOCELLUM
ETHANOL
PRODUCTION
CELLULOLYTIC ACTIVITY
CELLULOSE
CHEMICAL PREPARATION
FEASIBILITY STUDIES
ALCOHOLS
BACTERIA
CARBOHYDRATES
CHEMICAL REACTIONS
CLOSTRIDIUM
DECOMPOSITION
ENZYME ACTIVITY
ENZYMES
GLYCOSYL HYDROLASES
HYDROLASES
HYDROLYSIS
HYDROXY COMPOUNDS
LYSIS
MICROORGANISMS
O-GLYCOSYL HYDROLASES
ORGANIC COMPOUNDS
POLYSACCHARIDES
SACCHARIDES
SOLVOLYSIS
SYNTHESIS
090222* - Alcohol Fuels- Preparation from Wastes or Biomass- (1976-1989)
140504 - Solar Energy Conversion- Biomass Production & Conversion- (-1989)