cDNA sequence of human transforming gene hst and identification of the coding sequence required for transforming activity
The hst gene was originally identified as a transforming gene in DNAs from human stomach cancers and from a noncancerous portion of stomach mucosa by DNA-mediated transfection assay using NIH3T3 cells. cDNA clones of hst were isolated from the cDNA library constructed from poly(A)/sup +/ RNA of a secondary transformant induced by the DNA from a stomach cancer. The sequence analysis of the hst cDNA revealed the presence of two open reading frames. When this cDNA was inserted into an expression vector containing the simian virus 40 promoter, it efficiently induced the transformation of NIH3T3 cells upon transfection. It was found that one of the reading frames, which coded for 206 amino acids, was responsible for the transforming activity.
- Research Organization:
- National Cancer Center Research Institute, Tokyo, Japan
- OSTI ID:
- 5964664
- Journal Information:
- Proc. Natl. Acad. Sci. U.S.A.; (United States), Vol. 84:9
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
PROTEINS
AMINO ACID SEQUENCE
RECOMBINANT DNA
DNA SEQUENCING
BACTERIOPHAGES
ESCHERICHIA COLI
HYBRIDIZATION
MAN
MUCOUS MEMBRANES
ONCOGENES
ONCOGENIC TRANSFORMATIONS
PHOSPHORUS 32
STOMACH
ANIMALS
BACTERIA
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
CELL TRANSFORMATIONS
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
DNA
GASTROINTESTINAL TRACT
GENES
ISOTOPES
LIGHT NUCLEI
MAMMALS
MEMBRANES
MICROORGANISMS
MOLECULAR STRUCTURE
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANS
PARASITES
PHOSPHORUS ISOTOPES
PRIMATES
RADIOISOTOPES
STRUCTURAL CHEMICAL ANALYSIS
VERTEBRATES
VIRUSES
550201* - Biochemistry- Tracer Techniques