Effects of mycoplasma contamination on phenotypic expression of mitochondrial mutants in human cells
HeLa cells sensitive to the mitochondrial protein synthesis inhibitors erythromycin (ERY) and chloramphenicol (CAP) and HeLa variants resistant to the effects of these drugs were purposefully infected with drug-sensitive and -resistnat mycoplasma strains. Mycoplasma hyorhinis and the ERY-resistant strain of Mycoplasma orale, MO-ERY/sup r/, did not influence the growth of HeLa and ERY-resistant ERY2301 cells in the presence or absence of ERY. M. hyorhinis also did not affect the growth of HeLa and CAP-resistant Cap-2 cells in the presence or absence of CAP. However, both HeLa and Cap-2 cells infected with the CAP-resistant strain of M. hyorhinis, MH-CAP/sup r/, were more sensitive to the cytotoxix effect of CAP. This maybe due to the glucose dependence of the cells, which was compromised by the increased utilization of glucose by MH-CAP/sup r/ in these infected cell cultures. In vitro protein synthesis by isolated mitochondria was significantly altered by mycoplasma infection of the various cell lines. A substantial number of mycoplasmas copurified with the mitochondria, resulting in up to a sevenfold increase in the incorporation of (/sup 3/H)leucine into the trichloroacetic acid-insoluble material. More importantly, the apparent drug sensitivity or resistance of mitchondrial preparations from mycoplasma-infected cells reflected the drug sensitivity or resistance of the contaminating mycoplasmas. These results illustrate the hazards in interpreting mitochondrial protein synthesis data derived from mycoplasma-infected cell lines, particularly putative mitochondrially encoded mutants resistant to inhibitors of mitochondrial protein synthesis.
- Research Organization:
- Div. of Biology, California Institute of Technology, Pasadena, CA 91125
- OSTI ID:
- 5963475
- Journal Information:
- Mol. Cell. Biol.; (United States), Vol. 1:4
- Country of Publication:
- United States
- Language:
- English
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CHLORAMPHENICOL
METABOLISM
ERYTHROMYCIN
MITOCHONDRIA
MOLECULAR BIOLOGY
STRUCTURE-ACTIVITY RELATIONSHIPS
MYCOPLASMA
BIOLOGICAL PATHWAYS
SENSITIVITY
BIOLOGICAL EVOLUTION
CELL CULTURES
CELL PROLIFERATION
GLUCOSE
HELA CELLS
IN VITRO
LEUCINE
MAN
PHENOTYPE
TISSUE EXTRACTS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ALDEHYDES
AMINO ACIDS
ANIMALS
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
BIOLOGICAL MATERIALS
CARBOHYDRATES
CARBOXYLIC ACIDS
CELL CONSTITUENTS
DRUGS
HEXOSES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
MATERIALS
MICROORGANISMS
MONOSACCHARIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANOIDS
PRIMATES
SACCHARIDES
VERTEBRATES
550501* - Metabolism- Tracer Techniques
550701 - Microbiology- Tracer Techniques