skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Phosphoinositides in barley aleurone layers and gibberellic acid-induced changes in metabolism

Abstract

Phospholipids of barley (Hordeum vulgare L. cv Himalaya) aleurone layers were labeled with myo-(2-{sup 3}H)inositol or ({sup 32}Pi), extracted, and analyzed by physical (chromatography) and chemical (deacylation) techniques. Three phospholipids were found to incorporate both myo-(2-{sup 3}H)inositol and ({sup 32}Pi)-phosphatidylinositol, phosphatidylinositol-monophosphate, and phosphatidylinositol-bisphosphate. Stimulation of ({sup 3}H)inositol prelabeled aleurone layers with GA{sub 3} showed enhanced incorporation of label into phosphatidylinositol within 30 seconds and subsequent rapid breakdown. Stimulation of phosphatidylinositol labeling observed in these studies is the earliest response of aleurone cells to gibberellic acid reported.

Authors:
; ;  [1]
  1. Michigan Technological Univ., Houghton (USA)
Publication Date:
OSTI Identifier:
5962288
Resource Type:
Journal Article
Journal Name:
Plant Physiology; (USA)
Additional Journal Information:
Journal Volume: 91:4; Journal ID: ISSN 0032-0889
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; PHOSPHOLIPIDS; BIOCHEMICAL REACTION KINETICS; BARLEY; GIBBERELLIC ACID; INOSITOLS; PHOSPHORUS 32; TRACER TECHNIQUES; TRITIUM COMPOUNDS; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; CARBOHYDRATES; CARBOXYLIC ACIDS; CEREALS; DAYS LIVING RADIOISOTOPES; ESTERS; GRASS; HETEROCYCLIC COMPOUNDS; HYDROGEN COMPOUNDS; HYDROXY ACIDS; ISOTOPE APPLICATIONS; ISOTOPES; KINETICS; LACTONES; LIGHT NUCLEI; LILIOPSIDA; LIPIDS; MAGNOLIOPHYTA; MONOSACCHARIDES; NUCLEI; ODD-ODD NUCLEI; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANIC PHOSPHORUS COMPOUNDS; PHOSPHORUS ISOTOPES; PLANTS; RADIOISOTOPES; REACTION KINETICS; SACCHARIDES; 550201* - Biochemistry- Tracer Techniques

Citation Formats

Murthy, P P.N., Renders, J M, and Keranen, L M. Phosphoinositides in barley aleurone layers and gibberellic acid-induced changes in metabolism. United States: N. p., 1989. Web. doi:10.1104/pp.91.4.1266.
Murthy, P P.N., Renders, J M, & Keranen, L M. Phosphoinositides in barley aleurone layers and gibberellic acid-induced changes in metabolism. United States. https://doi.org/10.1104/pp.91.4.1266
Murthy, P P.N., Renders, J M, and Keranen, L M. 1989. "Phosphoinositides in barley aleurone layers and gibberellic acid-induced changes in metabolism". United States. https://doi.org/10.1104/pp.91.4.1266.
@article{osti_5962288,
title = {Phosphoinositides in barley aleurone layers and gibberellic acid-induced changes in metabolism},
author = {Murthy, P P.N. and Renders, J M and Keranen, L M},
abstractNote = {Phospholipids of barley (Hordeum vulgare L. cv Himalaya) aleurone layers were labeled with myo-(2-{sup 3}H)inositol or ({sup 32}Pi), extracted, and analyzed by physical (chromatography) and chemical (deacylation) techniques. Three phospholipids were found to incorporate both myo-(2-{sup 3}H)inositol and ({sup 32}Pi)-phosphatidylinositol, phosphatidylinositol-monophosphate, and phosphatidylinositol-bisphosphate. Stimulation of ({sup 3}H)inositol prelabeled aleurone layers with GA{sub 3} showed enhanced incorporation of label into phosphatidylinositol within 30 seconds and subsequent rapid breakdown. Stimulation of phosphatidylinositol labeling observed in these studies is the earliest response of aleurone cells to gibberellic acid reported.},
doi = {10.1104/pp.91.4.1266},
url = {https://www.osti.gov/biblio/5962288}, journal = {Plant Physiology; (USA)},
issn = {0032-0889},
number = ,
volume = 91:4,
place = {United States},
year = {Fri Dec 01 00:00:00 EST 1989},
month = {Fri Dec 01 00:00:00 EST 1989}
}