The bovine peripheral-type benzodiazepine receptor: A receptor with low affinity for benzodiazepines

Journal Article · Tue Jan 01 00:00:00 EST 1991 · Life Sciences; (USA)

DOI:https://doi.org/10.1016/0024-3205(91)90090-X· OSTI ID:5961858

Parola, A L; Laird, II, H E ^[1]

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1. Univ. of Arizona, Tucson (USA)

The density of bovine peripheral-type benzodiazepine receptors (PBR) in four tissues was highest in adrenal cortex. The adrenal cortex PBR cofractionated with a mitochondrial membrane marker enzyme and could be solubilized with intact ligand binding properties using digitonin. The membrane bound and soluble mitochondrial receptors were pharmacologically characterized and showed the rank order of potency to inhibit ({sup 3}H)PK 11195 binding was PK 11195 > protoporphyrin IX > benzodiazepines. ({sup 3}H)PK 11195 binding to bovine adrenal mitochondria was unaffected by diethylpyrocarbonate, a histidine residue modifying reagent that decreased binding to rat liver mitochondria by 70%. ({sup 3}H)PK 14105 photolabeled the bovine PBR and the Mr was estimated under nondenaturing and denaturing conditions. These results demonstrate the bovine peripheral-type benzodiazepine receptor is pharmacologically and biochemically distinct from the rat receptor, but the receptor component photolabeled by an isoquinoline ligand has a similar molecular weight.

• OSTI ID: 5961858
• Journal Information: Life Sciences; (USA), Vol. 48:8; ISSN 0024-3205
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
RECEPTORS
CHEMICAL COMPOSITION
SYMPATHOMIMETICS
ADRENAL GLANDS
AFFINITY
BIOCHEMICAL REACTION KINETICS
CATTLE
CELL MEMBRANES
DENSITY
MITOCHONDRIA
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ANIMALS
AUTONOMIC NERVOUS SYSTEM AGENTS
BODY
CELL CONSTITUENTS
DOMESTIC ANIMALS
DRUGS
ENDOCRINE GLANDS
GLANDS
HYDROGEN COMPOUNDS
ISOTOPE APPLICATIONS
KINETICS
MAMMALS
MEMBRANE PROTEINS
MEMBRANES
ORGANIC COMPOUNDS
ORGANOIDS
ORGANS
PHYSICAL PROPERTIES
PROTEINS
REACTION KINETICS
RUMINANTS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques