Kinetics of intralymphatically delivered monoclonal antibodies
Radiolabeled monoclonal antibody (MoAb) administration subcutaneously (sq), so that preferential uptake is to the lymphatics, holds significant promise for the detection of lymph node metastases. Only limited information is available about clearance rates of intralymphatically administered MoAbs. I-131 labeled intact IgG (225.28S), F(ab's)2 (225.28S) or IgM (FT162) were administered sq to anesthetized Balb/C mice. Eight mice were studied with each MoAb, 4 with a foot-pad injection, 4 with an anterior abdominal injection. Gamma camera images were collected into a computer, over the first 6 hrs after injection with the animals anesthetized and immobile. Animals were then allowed to move about freely. Additional images were then acquired out to 48 hrs. Regions of interest wre selected over the injection site and the kinetics of antibody egress determined. Clearance rates from local sq injection sites are influenced by motion and somewhat by location. The class and fragment status of the MoAb appear relatively less important in determining clearance rates from sq injections than they are in determining whole-body clearance after iv injections. Additional studies using Fab fragments and additional monoclonals will be useful in extending these observations.
- Research Organization:
- Univ. of Michigan Medical Center, Ann Arbor, MI
- OSTI ID:
- 5960606
- Report Number(s):
- CONF-850611-; TRN: 87-039295
- Journal Information:
- J. Nucl. Med.; (United States), Vol. 26:5; Conference: 32. annual meeting of the Society of Nuclear Medicine, Houston, TX, USA, 2 Jun 1985
- Country of Publication:
- United States
- Language:
- English
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LYMPHOMAS
DIAGNOSIS
RADIOIMMUNOSCINTIGRAPHY
MONOCLONAL ANTIBODIES
DIAGNOSTIC USES
LABELLING
TISSUE DISTRIBUTION
ABDOMEN
CLEARANCE
IODINE 131
LYMPHATIC SYSTEM
MICE
RETENTION FUNCTIONS
SUBCUTANEOUS INJECTION
UPTAKE
ANIMALS
ANTIBODIES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
BODY AREAS
COUNTING TECHNIQUES
DAYS LIVING RADIOISOTOPES
DIAGNOSTIC TECHNIQUES
DISEASES
DISTRIBUTION
FUNCTIONS
INJECTION
INTAKE
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPES
MAMMALS
NEOPLASMS
NUCLEI
ODD-EVEN NUCLEI
RADIOISOTOPE SCANNING
RADIOISOTOPES
RODENTS
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VERTEBRATES
550601* - Medicine- Unsealed Radionuclides in Diagnostics