/sup 13/C and /sup 61/Ni isotope substitutions confirm the presence of a nickel(III)-carbon species in acetogenic CO dehydrogenases
The nickel-containing CO dehydrogenase from Acetobacterium woodii and Clostridium thermoaceticum were studied by EPR spectroscopy in order to define the components involved in the EPR spectrum obtained by reaction of the enzymes with the substrate, CO. Using isotopic substitution techniques, these experiments unequivocally establish that a nickel-carbon species is involved in the g=2.08, 2.02 EPR signal. Comparing the /sup 61/Ni- and /sup 59/Ni-substituted enzymes, the g=2.08 component of the resonance was found to be mainly due to nickel with a smaller contribution by the carbon species. Reaction of the CO dehydrogenase with (/sup 13/C)CO versus (/sup 12/C)CO showed that a carbon species, formed from CO, was the major contributor to the g=2.02 EPR signal. In addition, the oxidized CO dehydrogenase was found to exhibit a Ni(III) EPR signal analogous to that of the hydrogenase from the methanogenic and sulfate-reducing bacteria. 19 references, 3 figures.
- Research Organization:
- Univ. of Georgia, Athens
- DOE Contract Number:
- AS09-79ER10499
- OSTI ID:
- 5960072
- Journal Information:
- Biochem. Biophys. Res. Commun.; (United States), Vol. 115:2
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
OXIDOREDUCTASES
ELECTRON SPIN RESONANCE
STRUCTURAL CHEMICAL ANALYSIS
BIOCHEMICAL REACTION KINETICS
CARBON 12
CARBON 13
CARBON MONOXIDE
CLOSTRIDIUM
ENZYME ACTIVITY
LABELLED COMPOUNDS
METHANOGENIC BACTERIA
NICKEL 59
NICKEL 61
SULFATE-REDUCING BACTERIA
TRACER TECHNIQUES
BACTERIA
BETA DECAY RADIOISOTOPES
CARBON COMPOUNDS
CARBON ISOTOPES
CARBON OXIDES
CHALCOGENIDES
ELECTRON CAPTURE RADIOISOTOPES
ENZYMES
EVEN-EVEN NUCLEI
EVEN-ODD NUCLEI
INTERMEDIATE MASS NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LIGHT NUCLEI
MAGNETIC RESONANCE
MICROORGANISMS
NICKEL ISOTOPES
NUCLEI
OXIDES
OXYGEN COMPOUNDS
RADIOISOTOPES
REACTION KINETICS
RESONANCE
STABLE ISOTOPES
YEARS LIVING RADIOISOTOPES
560301* - Chemicals Metabolism & Toxicology- Cells- (-1987)
550201 - Biochemistry- Tracer Techniques