Comparative biochemical characterization of a human IgM produced in both ascites and in vitro cell culture
- Stanford Univ., CA (United States)
Researchers have conducted a comparative analysis of a monoclonal human IgM obtained from cells cultured in nude-mouse ascites and from the same cells cultured in a bioreactor. We studied the glycosylation of the IgMs using lectin blotting and high-pH anion-exchange chromatography with pulsed amperometric detection (HPAE-PAD), and we also developed reverse phase liquid chromatography (RPLC) peptide maps of the IgM samples. The HPAE-PAD data indicate that the samples differ in both the type and distribution of oligosaccharides present on the IgMs. In addition, the proteins differ in their solubility behavior and in their RPLC peptide maps. We conclude that the method of cell culture of capable of significantly altering the characteristics of the glycoprotein product. 27 refs., 5 figs.
- OSTI ID:
- 5954279
- Journal Information:
- Bio/Technology; (United States), Vol. 11:4; ISSN 0733-222X
- Country of Publication:
- United States
- Language:
- English
Similar Records
Glycosylation of the T-cell antigen-specific receptor and its potential role in lectin-mediated cytotoxicity
Biochemical characterization of domain-specific glycoproteins of the rat hepatocyte plasma membrane