Fractionated irradiation and early changes in salivary glands. Different effects on potassium efflux, exocytotic amylase release and gland morphology

Journal Article · Fri Feb 01 00:00:00 EST 1991 · Laboratory Investigation (A Journal of Experimental Methods and Pathology); (USA)

OSTI ID:5952111

Franzen, L; Funegard, U S; Sundstroem, S G; Gustafsson, H; Danielsson, A; Henriksson, R ^[1]

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1. University Hospital, Umea (Sweden)

Irradiation is a potent treatment modality of head and neck cancer. However, the irradiation is usually associated with an influence on salivary glands with ensuing dryness and discomfort for the patients. In the present study we used different in vitro secretory models and morphologic characterization of rat parotid gland. Radiation was given to one gland on a 5-day schedule with 6 MV photons (total dose 20, 30, 35, 40, 45 Gy). The contralateral gland served as control, and the analysis of glands were performed 10 days after the last irradiation treatment. The noradrenaline stimulated electrolyte secretion (86rubidium tracer for potassium) was decreased in relation to the irradiation dose and in comparison to contralateral control glands. Noradrenaline stimulated exocytotic amylase release was not affected by irradiation and, there were no signs of obvious quantitative morphologic alterations after irradiation compared with controls. The results suggest that there are differences in the sensitivity to radiation for the two different secretory processes in salivary glands, and, thus, the structures regulating electrolyte and fluid secretion seem to be more vulnerable to irradiation than the process of exocytosis. The results, however, do not allow discrimination between temporary cellular impairment and irreversible damage leading to cell death.

• OSTI ID: 5952111
• Journal Information: Laboratory Investigation (A Journal of Experimental Methods and Pathology); (USA), Vol. 64:2; ISSN 0023-6837
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
59 BASIC BIOLOGICAL SCIENCES
AMYLASE
SECRETION
POTASSIUM
MEMBRANE TRANSPORT
RADIOTHERAPY
SIDE EFFECTS
SALIVARY GLANDS
BIOLOGICAL RADIATION EFFECTS
FRACTIONATED IRRADIATION
MORPHOLOGY
NORADRENALINE
RADIATION DOSES
RATS
RUBIDIUM 86
TRACER TECHNIQUES
ADRENAL HORMONES
ALKALI METAL ISOTOPES
ALKALI METALS
ANIMALS
AUTONOMIC NERVOUS SYSTEM AGENTS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BODY
CARDIOTONICS
CARDIOVASCULAR AGENTS
DAYS LIVING RADIOISOTOPES
DOSES
DRUGS
ELEMENTS
ENZYMES
GLANDS
GLYCOSYL HYDROLASES
HYDROLASES
INTERMEDIATE MASS NUCLEI
IRRADIATION
ISOMERIC TRANSITION ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
MEDICINE
METALS
MINUTES LIVING RADIOISOTOPES
NEUROREGULATORS
NUCLEAR MEDICINE
NUCLEI
O-GLYCOSYL HYDROLASES
ODD-ODD NUCLEI
ORGANS
RADIATION EFFECTS
RADIOISOTOPES
RADIOLOGY
RODENTS
RUBIDIUM ISOTOPES
SYMPATHOMIMETICS
THERAPY
VERTEBRATES
560152* - Radiation Effects on Animals- Animals
550201 - Biochemistry- Tracer Techniques