IL-3 specifically inhibits GM-CSF binding to the higher affinity receptor
Abstract
The inhibition of binding between human granulocyte-macrophage colony-stimulating factor (GM-CSF) and its receptor by human interleukin-3 (IL-3) was observed in myelogenous leukemia cell line KG-1 which bore the receptors both for GM-CSF and IL-3. In contrast, this phenomenon was not observed in histiocytic lymphoma cell line U-937 or in gastric carcinoma cell line KATO III, both of which have apparent GM-CSF receptor but an undetectable IL-3 receptor. In KG-1 cells, the cross-inhibition was preferentially observed when the binding of GM-CSF was performed under the high-affinity binding condition; i.e., a low concentration of 125I-GM-CSF was incubated. Scatchard analysis of 125I-GM-CSF binding to KG-1 cells in the absence and in the presence of unlabeled IL-3 demonstrated that IL-3 inhibited GM-CSF binding to the higher-affinity component of GM-CSF receptor on KG-1 cells. Moreover, a chemical cross-linking study has revealed that the cross-inhibition of the GM-CSF binding observed in KG-1 cells is specific for the beta-chain, Mr 135,000 binding protein which has been identified as a component forming the high-affinity GM-CSF receptor existing specifically on hemopoietic cells.
- Authors:
-
- Univ. of Tokyo (Japan)
- Publication Date:
- OSTI Identifier:
- 5945756
- Resource Type:
- Journal Article
- Journal Name:
- Journal of Cellular Physiology; (USA)
- Additional Journal Information:
- Journal Volume: 146:2; Journal ID: ISSN 0021-9541
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; GROWTH FACTORS; BIOCHEMICAL REACTION KINETICS; LYMPHOKINES; AFFINITY; CARCINOMAS; COLONY FORMATION; INHIBITION; IODINE 125; LEUKEMIA; LYMPHOMAS; MACROPHAGES; MAN; REAGENTS; RECEPTORS; TRACER TECHNIQUES; TUMOR CELLS; ANIMAL CELLS; ANIMALS; BETA DECAY RADIOISOTOPES; CONNECTIVE TISSUE CELLS; DAYS LIVING RADIOISOTOPES; DISEASES; ELECTRON CAPTURE RADIOISOTOPES; HEMIC DISEASES; IMMUNE SYSTEM DISEASES; INTERMEDIATE MASS NUCLEI; IODINE ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; KINETICS; MAMMALS; MEMBRANE PROTEINS; MITOGENS; NEOPLASMS; NUCLEI; ODD-EVEN NUCLEI; ORGANIC COMPOUNDS; PHAGOCYTES; PRIMATES; PROTEINS; RADIOISOTOPES; REACTION KINETICS; SOMATIC CELLS; VERTEBRATES; 550201* - Biochemistry- Tracer Techniques; 550901 - Pathology- Tracer Techniques
Citation Formats
Taketazu, F, Chiba, S, Shibuya, K, Kuwaki, T, Tsumura, H, Miyazono, K, Miyagawa, K, and Takaku, F. IL-3 specifically inhibits GM-CSF binding to the higher affinity receptor. United States: N. p., 1991.
Web. doi:10.1002/jcp.1041460209.
Taketazu, F, Chiba, S, Shibuya, K, Kuwaki, T, Tsumura, H, Miyazono, K, Miyagawa, K, & Takaku, F. IL-3 specifically inhibits GM-CSF binding to the higher affinity receptor. United States. https://doi.org/10.1002/jcp.1041460209
Taketazu, F, Chiba, S, Shibuya, K, Kuwaki, T, Tsumura, H, Miyazono, K, Miyagawa, K, and Takaku, F. 1991.
"IL-3 specifically inhibits GM-CSF binding to the higher affinity receptor". United States. https://doi.org/10.1002/jcp.1041460209.
@article{osti_5945756,
title = {IL-3 specifically inhibits GM-CSF binding to the higher affinity receptor},
author = {Taketazu, F and Chiba, S and Shibuya, K and Kuwaki, T and Tsumura, H and Miyazono, K and Miyagawa, K and Takaku, F},
abstractNote = {The inhibition of binding between human granulocyte-macrophage colony-stimulating factor (GM-CSF) and its receptor by human interleukin-3 (IL-3) was observed in myelogenous leukemia cell line KG-1 which bore the receptors both for GM-CSF and IL-3. In contrast, this phenomenon was not observed in histiocytic lymphoma cell line U-937 or in gastric carcinoma cell line KATO III, both of which have apparent GM-CSF receptor but an undetectable IL-3 receptor. In KG-1 cells, the cross-inhibition was preferentially observed when the binding of GM-CSF was performed under the high-affinity binding condition; i.e., a low concentration of 125I-GM-CSF was incubated. Scatchard analysis of 125I-GM-CSF binding to KG-1 cells in the absence and in the presence of unlabeled IL-3 demonstrated that IL-3 inhibited GM-CSF binding to the higher-affinity component of GM-CSF receptor on KG-1 cells. Moreover, a chemical cross-linking study has revealed that the cross-inhibition of the GM-CSF binding observed in KG-1 cells is specific for the beta-chain, Mr 135,000 binding protein which has been identified as a component forming the high-affinity GM-CSF receptor existing specifically on hemopoietic cells.},
doi = {10.1002/jcp.1041460209},
url = {https://www.osti.gov/biblio/5945756},
journal = {Journal of Cellular Physiology; (USA)},
issn = {0021-9541},
number = ,
volume = 146:2,
place = {United States},
year = {Fri Feb 01 00:00:00 EST 1991},
month = {Fri Feb 01 00:00:00 EST 1991}
}