Expression of. beta. -conglycinin gene driven by CaMV /sup 35/S promoter in transgenic plants
..beta..-conglycinin is a abundant protein stored in protein bodies of soybean seeds. This protein consists of three major subunits, ..cap alpha..' (76 kDa), ..cap alpha.. (72 kDa) and ..beta.. (53 kDa), and accumulates in developing soybean embryos during the mid- to late-maturation stages of seed development. Coding sequence of an ..cap alpha..'-subunit gene was expressed in transgenic petunia plants under control of the promoter from the CaMV (cauliflower mosaic virus) /sup 35/S transcript. Two different types of ..cap alpha..'-protein accumulated in tissues of the transgenic plant; seed-type ..cap alpha..'-protein accumulated only in seeds during mid- to late-maturation stages, while non-seed-type ..cap alpha..'-protein was found in non-seed tissues and in early stages of seed maturation. Seed-type ..cap alpha..'-protein was the same size as soybean ..cap alpha..'-subunit, while non-seed-type ..cap alpha..'-protein was larger by about 4 kDa. Seeds contained approximately 30-fold greater levels of ..cap alpha..'-protein than did non-seed tissues. This is presumably due to differences in protein stability because the amount of ..cap alpha..'-mRNA was equivalent in each of the tissues examined. The ..cap alpha..'-protein in leaves was localized in microsomal membrane fractions. Proteins solubilized from the membranes were sedimented by sucrose gradient centrifugation and analyzed by immuno blot technique. The results suggest that the protein assembles into multimeric forms in leaf membranes, as it does in seed protein bodies.
- Research Organization:
- Washington Univ., St. Louis, MO
- OSTI ID:
- 5942522
- Report Number(s):
- CONF-8707108-; TRN: 87-039112
- Journal Information:
- Plant Physiol., Suppl.; (United States), Vol. 83:4; Conference: Annual meeting of the American Society of Plant Physiologists, St. Louis, MO, USA, 19 Jul 1987
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ORNAMENTAL PLANTS
GENETIC ENGINEERING
PROTEIN STRUCTURE
CENTRIFUGATION
GENE REPRESSORS
IMMUNE REACTIONS
LABELLED COMPOUNDS
MEMBRANES
MESSENGER-RNA
PROTEINS
SEEDS
SOYBEANS
SULFUR 35
TISSUE DISTRIBUTION
TRACER TECHNIQUES
TRANSCRIPTION
VIRUSES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
DAYS LIVING RADIOISOTOPES
DISTRIBUTION
EVEN-ODD NUCLEI
FOOD
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MICROORGANISMS
NUCLEI
NUCLEIC ACIDS
NUCLEOPROTEINS
ORGANIC COMPOUNDS
PARASITES
PLANTS
RADIOISOTOPES
RNA
SEPARATION PROCESSES
SULFUR ISOTOPES
VEGETABLES
550201* - Biochemistry- Tracer Techniques
550401 - Genetics- Tracer Techniques