Cell surface of sea urchin micromeres and primary mesenchyme. [Arbacia punctulata; Strongylocentrotus drobachiensis; Strongylocentrotus purpuratus]
The cell surface and extracellular matrix (ECM) of the sea urchin embryo were studied during the early morphogenetic events involved in the differentiation of the micromere cell lineage. Sixteen-cell and early cleavage stage blastomeres were isolated and the protein composition of their cell surfaces examined by /sup 125/I-labelling followed by SDS-polyacrylamide gel electrophoresis (SDS-PAGE). Micromere-specific cell surface proteins are reported for Arbacia punctulata, Strongylocentrotus droebachiensis, and Strongylocentrotus purpuratus. Cell surface glycoproteins were characterized on the basis of lectin binding specificity with a novel lectin affinity transfer technique. Using this procedure, cell-type specific surface proteins, which are also lectin-binding specific, can be detected. In addition, fluorescein conjugated lectins were microinjected into the blastocoels of living S. drobachiensis and Lytechinus pictus embryos and the patterns of lectin bindings observed by fluorescence microscopy. The evidence presented in this thesis suggests that the differentiation of the primary mesenchyme cells is correlated with changes in the molecular composition of the cell-surface and the ECM.
- Research Organization:
- Dartmouth Coll., Hanover, NH (USA)
- OSTI ID:
- 5941861
- Resource Relation:
- Other Information: Thesis (Ph. D.)
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
EMBRYONIC CELLS
CHEMICAL COMPOSITION
GLUCOPROTEINS
ELECTROPHORESIS
SEA URCHINS
CELL DIFFERENTIATION
BIOCHEMICAL REACTION KINETICS
EMBRYOS
EXPERIMENTAL DATA
IODINE 125
LECTINS
PROTEINS
TRACER TECHNIQUES
ANIMAL CELLS
ANIMALS
AQUATIC ORGANISMS
BETA DECAY RADIOISOTOPES
CARBOHYDRATES
DATA
DAYS LIVING RADIOISOTOPES
ECHINODERMS
ELECTRON CAPTURE RADIOISOTOPES
INFORMATION
INTERMEDIATE MASS NUCLEI
INVERTEBRATES
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
NUCLEI
NUMERICAL DATA
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
RADIOISOTOPES
REACTION KINETICS
SACCHARIDES
550301* - Cytology- Tracer Techniques