Quantitation of gibberellins A sub 1 , A sub 3 , A sub 4 , A sub 9 and a putative A sub 9 -conjugate in grafts of Sitka spruce (Picea sitchensis) during the period of shoot elongation

Journal Article · Wed Aug 01 00:00:00 EDT 1990 · Plant Physiology; (USA)

DOI:https://doi.org/10.1104/pp.93.4.1476· OSTI ID:5941584

Moritz, T; Philipson, J J; Oden, P C ^[1]

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1. Swedish Univ. of Agricultural Sciences, Umea (Sweden) Forestry Commission Northern Research Station, Roslin Midlothian (England)

The levels of endogenous gibberellin A{sub 1}(GA{sub 1}), GA{sub 3}, GA{sub 4}, GA{sub 9}, and a cellulase hydrolyzable GA{sub 9} conjugate in needles and shoot stems of mature grafts of Sitka spruce (Picea sitchensis (Bong.) Carr.) grown under environmental conditions that were either inductive, hot, and dry, or noninductive, cool, and wet, for flowering, were estimated by combined gas chromatography-mass spectrometry selected ion monitoring using deuterated ({sup 2}H{sub 2})GA{sub 1}, GA{sub 3}, GA{sub 4}, and GA{sub 9} as internal standards. The results imply differential metabolism between clones treated with conditions inductive and noninductive for flowering. Higher concentrations of putative GA{sub 9} conjugate and free GA{sub 9} in the hot and dry treatment indicate a higher capacity of synthesizing, for flowering, the physiologically important GA{sub 4} in the heat and drought-treated material. This synthesis does not, however, result in a buildup of the GA{sub 4} pool, probably because of a high turnover rate of GA{sub 4}. The cool and wet-treated material had higher amounts of GA{sub 1} and GA{sub 3}, indicating that the differentiation was preferentially directed toward vegetative growth.

• OSTI ID: 5941584
• Journal Information: Plant Physiology; (USA), Vol. 93:4; ISSN 0032-0889
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
GIBBERELLIC ACID
MEASURING METHODS
SPRUCES
PLANT GROWTH
DEUTERIUM COMPOUNDS
DROUGHTS
ENVIRONMENT
MOISTURE
PHYSIOLOGY
TRACER TECHNIQUES
CARBOXYLIC ACIDS
CONIFERS
ESTERS
GROWTH
HETEROCYCLIC COMPOUNDS
HYDROGEN COMPOUNDS
HYDROXY ACIDS
ISOTOPE APPLICATIONS
LACTONES
ORGANIC ACIDS
ORGANIC COMPOUNDS
PINOPHYTA
PLANTS
TREES
551001* - Physiological Systems- Tracer Techniques