Development and evaluation of an anchorage-independent agar-based clonal assay for human primary breast carcinoma cells

Thesis/Dissertation · Tue Jan 01 00:00:00 EST 1985

OSTI ID:5939685

Besch, G J

The development and evaluation of an anchorage-independent clonal cytotoxic assay for primary human breast carcinoma cells is described in this thesis. This assay was developed in three stages which include: (1) the optimization of the production of a monodispersed cell suspension from solid breast carcinomas, (2) the systematic development of a growth medium for the clonal growth of these cells, and (3) the adaptation of these methods for use in the quantitation of cytotoxicity. The results of these studies indicated that hydrocortisone, fetal bovine serum and red blood cells stimulated the clonal growth of breast carcinoma cells. The optimal concentrations of these three factors were simultaneously determined using response surface methodology. These culture conditions were then used to develop radiation-cytotoxicity assays for both primary and recurrent breast carcinomas. The methodology developed and evaluated in this thesis may be useful to: (1) study the biology and radiobiology of human breast cancer, (2) customize the treatment of individual breast cancer patients, and (3) identify and/or develop new drugs and/or other treatment modalities for breast cancer.

• Research Organization: Wisconsin Univ., Madison (USA)
• OSTI ID: 5939685
• Resource Relation: Other Information: Thesis (Ph. D.)
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
TUMOR CELLS
BIOLOGICAL RADIATION EFFECTS
CYTOLOGICAL TECHNIQUES
BIOASSAY
CARCINOMAS
TOXICITY
ANIMAL CELLS
BIOLOGICAL EFFECTS
DISEASES
NEOPLASMS
RADIATION EFFECTS
560121* - Radiation Effects on Cells- External Source- (-1987)