One carbon metabolism in anaerobic bacteria. Organic acid and methane production. Progress report, June 1985-May 1986
Our project focussed on understanding of one and multicarbon metabolism in acetogenic and methanogenic bacteria. The studies with acetate catabolism pathway in Methanosarcina barkeri showed involvement of methyl reductase, methyl coenzyme M and carbon monoxide dehydrogenase in the proposed model of acetate catabolism. We also examined the relationship between hydrogen metabolism and methanogenesis, isotopic discrimination during methanogenesis, and sulfate dependent interspecies H/sub 2/-transfer between M. barkeri and Desulfovibrio vulgaris. Ammonia assimilation in Methanobacterium ivanovii via glutamine synthetase (GS) was confirmed by isolation of GS-deficient and GS-impared auxotrophic mutants. GS from M. ivanovii is also purified, characterized and compared with the eubacterial GS. In Butyribacterium methylotrophicum various enzymes involved in homoacetogenic catabolism of formate, formate plus CO and methanol plus CO were examined. Carbon dioxide was shown to be a required intermediate during catabolism of CO by B. methylotrophicum. Studies were also conducted for the operation of electron transport phosphorylation (ETP) in B. methylotrophicum by analyzing the proton motive force (PMF) and membrane bound electron carriers. 13 refs.
- Research Organization:
- Michigan State Univ., East Lansing (USA). Dept. of Biochemistry
- DOE Contract Number:
- FG02-85ER13376
- OSTI ID:
- 5932862
- Report Number(s):
- DOE/ER/13376-1; ON: DE86010584
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
09 BIOMASS FUELS
ACETIC ACID
METABOLISM
METHANOGENIC BACTERIA
BIOLOGICAL PATHWAYS
DESULFOVIBRIO
METHYL TRANSFERASES
OXIDOREDUCTASES
BACTERIA
CARBOXYLIC ACIDS
ENZYMES
MICROORGANISMS
MONOCARBOXYLIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
SULFATE-REDUCING BACTERIA
TRANSFERASES
550200* - Biochemistry
090122 - Hydrocarbon Fuels- Preparation from Wastes or Biomass- (1976-1989)