Formation of. beta. ,. gamma. -methylene-7,8-dihydroneopterin 3'-triphosphate from. beta. ,. gamma. -methyleneguanosine 5'-triphosphate by GTP cyclohydrolase I of Escherichia coli

Journal Article · Sun Jan 01 00:00:00 EST 1984 · Arch. Biochem. Biophys.; (United States)

DOI:https://doi.org/10.1016/0003-9861(84)90470-3· OSTI ID:5932805

Ferre, J; Jacobson, K B

GTP cyclohydrolase I of Escherichia coli converts (..beta..,..gamma..-methylene)GTP to a fluorescent product that is characterized as (..beta..,..gamma..-methylene)dihydroneopterin triphosphate. Interaction between the GTP analog and the enzyme gave a K/sub i/ of 3.0 ..mu..M, which may be compared to the K/sub m/ of 0.1 ..mu..M for GTP. This new analog of dihydroneopterin triphosphate may, in turn, be converted to the same greenish-yellow pteridines (compounds X, X1, and X2) that are obtained from dihydroneopterin triphosphate. Because of its stability to phosphatase action, this analog may be useful for studies in pteridine metabolism. 14 references, 5 figures.

• Research Organization: Oak Ridge National Lab., TN
• DOE Contract Number: AC05-84OR21400
• OSTI ID: 5932805
• Journal Information: Arch. Biochem. Biophys.; (United States), Vol. 233:2, Issue 2
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
HYDROLASES
ENZYME ACTIVITY
PHOSPHORIC ACID ESTERS
BIOSYNTHESIS
BIOCHEMICAL REACTION KINETICS
ESCHERICHIA COLI
EXPERIMENTAL DATA
BACTERIA
DATA
ENZYMES
ESTERS
INFORMATION
KINETICS
MICROORGANISMS
NUMERICAL DATA
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
REACTION KINETICS
SYNTHESIS
550200* - Biochemistry