Effect of protein diet on age-dependent methylation of liver nuclear components of mice administered N-nitrosodimethylamine

Klaude, M; von der Decken, A

Title: Effect of protein diet on age-dependent methylation of liver nuclear components of mice administered N-nitrosodimethylamine

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Abstract

Protein diets deficient or enriched in DL-methionine were studied for the effect of methylation of nuclear components in subadult and adult outbred albino male mice given N-nitrosodimethylamine. After the mice were fed diets for 6 days, they were given ip injections of (/sup 14/C)N-nitrosodimethylamine (5 mg/kg body wt) and killed 45 minutes later. Liver nuclei were isolated; incorporation of /sup 14/C into nuclear proteins and methylation of DNA and RNA were determined. In the subadult and adult animals the nuclear protein content was diminished by shortage of a single essential amino acid. Incorporation of /sup 14/C into the total protein was reduced. The reduction amounted to 55% in the subadult and 23% in the adult animals. Neither an age-dependent nor a diet-dependent change in the specific methylation of DNA and RNA was observed. Analysis of methylation of DNA and RNA was observed. Analysis of methylated purine bases showed an age-related rise in O6-methylguanine in the adult as compared with the subadult mice.

Authors:: Klaude, M; von der Decken, A

Publication Date:: Mon Oct 01 00:00:00 EDT 1984

Research Org.:: Univ. of Stockholm, Sweden

OSTI Identifier:: 5928054

Resource Type:: Journal Article

Journal Name:: J. Natl. Cancer Inst.; (United States)

Additional Journal Information:: Journal Volume: 73:4

Country of Publication:: United States

Language:: English

Subject:: 63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; DNA; METHYLATION; NITROSAMINES; BIOLOGICAL EFFECTS; RNA; AGE DEPENDENCE; AMINO ACIDS; CARBON 14 COMPOUNDS; DIET; LIVER; MICE; NUTRITIONAL DEFICIENCY; PROTEINS; RESPONSE MODIFYING FACTORS; TRACER TECHNIQUES; AMINES; ANIMALS; BODY; CARBOXYLIC ACIDS; CHEMICAL REACTIONS; DIGESTIVE SYSTEM; GLANDS; ISOTOPE APPLICATIONS; LABELLED COMPOUNDS; MAMMALS; NITROSO COMPOUNDS; NUCLEIC ACIDS; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; ORGANS; RODENTS; VERTEBRATES; 560305* - Chemicals Metabolism & Toxicology- Vertebrates- (-1987)

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                    Klaude, M, and von der Decken, A. Effect of protein diet on age-dependent methylation of liver nuclear components of mice administered N-nitrosodimethylamine.  United States: N. p., 1984. 
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                    Klaude, M, & von der Decken, A. Effect of protein diet on age-dependent methylation of liver nuclear components of mice administered N-nitrosodimethylamine.  United States.

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                    Klaude, M, and von der Decken, A. 1984.  
        "Effect of protein diet on age-dependent methylation of liver nuclear components of mice administered N-nitrosodimethylamine".  United States.

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@article{osti_5928054,

  title        = {Effect of protein diet on age-dependent methylation of liver nuclear components of mice administered N-nitrosodimethylamine},

  author       = {Klaude, M and von der Decken, A},

  abstractNote = {Protein diets deficient or enriched in DL-methionine were studied for the effect of methylation of nuclear components in subadult and adult outbred albino male mice given N-nitrosodimethylamine. After the mice were fed diets for 6 days, they were given ip injections of (/sup 14/C)N-nitrosodimethylamine (5 mg/kg body wt) and killed 45 minutes later. Liver nuclei were isolated; incorporation of /sup 14/C into nuclear proteins and methylation of DNA and RNA were determined. In the subadult and adult animals the nuclear protein content was diminished by shortage of a single essential amino acid. Incorporation of /sup 14/C into the total protein was reduced. The reduction amounted to 55% in the subadult and 23% in the adult animals. Neither an age-dependent nor a diet-dependent change in the specific methylation of DNA and RNA was observed. Analysis of methylation of DNA and RNA was observed. Analysis of methylated purine bases showed an age-related rise in O6-methylguanine in the adult as compared with the subadult mice.},

  doi          = {},

  url          = {https://www.osti.gov/biblio/5928054},
  journal      = {J. Natl. Cancer Inst.; (United States)},
number       = ,

  volume       = 73:4,

  place        = {United States},

  year         = {Mon Oct 01 00:00:00 EDT 1984},

  month        = {Mon Oct 01 00:00:00 EDT 1984}

}

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